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Cranial and trunk neural crest cells use different mechanisms for attachment to extracellular matrices

Lallier, Thomas and Leblanc, Gabrielle and Artinger, Kristin B. and Bronner, Marianne E. (1992) Cranial and trunk neural crest cells use different mechanisms for attachment to extracellular matrices. Development, 116 (3). pp. 531-541. ISSN 0950-1991. http://resolver.caltech.edu/CaltechAUTHORS:20120315-154915681

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Abstract

We have used a quantitative cell attachment assay to compare the interactions of cranial and trunk neural crest cells with the extracellular matrix (ECM) molecules fibronectin, laminin and collagen types I and IV. Antibodies to the β_1 subunit of integrin inhibited attachment under all conditions tested, suggesting that integrins mediate neural crest cell interactions with these ECM molecules. The HNK-1 antibody against a surface carbohydrate epitope under certain conditions inhibited both cranial and trunk neural crest cell attachment to laminin, but not to fibronectin. An antiserum to α_1 intergrin inhibited attachment of trunk, but not cranial, neural crest cells to laminin and collagen type I, though interactions with fibronectin or collagen type IV were unaffected. The surface properties of trunk and cranial neural crest cells differed in several ways. First, trunk neural crest cells attached to collagen types I and IV, but cranial neural crest cells did not. Second, their divalent cation requirements for attachment to ECM molecules differed. For fibronectin substrata, trunk neural crest cells required divalent cations for attachment, whereas cranial neural crest cells bound in the absence of divalent cations. However, cranial neural crest cells lost this cation-independent attachment after a few days of culture. For laminin substrata, trunk cells used two integrins, one divalent cation-dependent and the other divalent cation-independent (Lallier, T. E. and Bronner-Fraser, M. (1991) Development 113, 1069–1081). In contrast, cranial neural crest cells attached to laminin using a single, divalent cation-dependent receptor system. Immunoprecipitations and immunoblots of surface labelled neural crest cells with HNK-1, α_1 integrin and β_1 integrin antibodies suggest that cranial and trunk neural crest cells possess biochemically distinct integrins. Our results demonstrate that cranial and trunk cells differ in their mechanisms of adhesion to selected ECM components, suggesting that they are non-overlapping populations of cells with regard to their adhesive properties.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dev.biologists.org/content/116/3/531PublisherUNSPECIFIED
Additional Information:© 1992 The Company of Biologists Limited. Accepted 19 August 1992. This work was supported by USPHS grant HD-15527 to M. B. F. and BNS-8702512 to G. G. L.
Funders:
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)HD-15527
U.S. Public Health Service (USPHS)BNS-8702512
Subject Keywords:integrins, cell adhesion, laminin, fibronectin, collagen
Record Number:CaltechAUTHORS:20120315-154915681
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20120315-154915681
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:29737
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:20 Mar 2012 21:40
Last Modified:26 Dec 2012 14:57

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