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Genetic organization of methylamine utilization genes from Methylobacterium extorquens AM1

Chistoserdov, Andrei Y. and Tsygankov, Yuri D. and Lidstrom, Mary E. (1991) Genetic organization of methylamine utilization genes from Methylobacterium extorquens AM1. Journal of Bacteriology, 173 (18). pp. 5901-5908. ISSN 0021-9193. http://resolver.caltech.edu/CaltechAUTHORS:CHIjbact91a

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Abstract

An isolated 5.2-kb fragment of Methylobacterium extorquens AM1 DNA was found to contain a gene cluster involved in methylamine utilization. Analysis of polypeptides synthesized in an Escherichia coli T7 expression system showed that five genes were present. Two of the genes encoded the large and small subunits of methylamine dehydrogenase, and a third encoded amicyanin, the presumed electron acceptor for methylamine dehydrogenase, but the function of the other two genes is not known. The order on the 5.2-kb fragment was found to be large-subunit gene, the two genes of unknown function, small-subunit gene, amicyanin gene. The gene for azurin, another possible electron acceptor in methylamine oxidation, does not appear to be present within this cluster of methylamine utilization genes.


Item Type:Article
Additional Information:Copyright © 1991 American Society for Microbiology. Received 15 February 1991. Accepted 25 June 1991. This work was supported by a grant from the NIH (GM 36296). We thank Chris Anthony for supplying antibody to cytochrome c-553.
Subject Keywords:BLUE COPPER PROTEINS; SP STRAIN AM1; PSEUDOMONAS SP-J; PARACOCCUS-DENITRIFICANS; OBLIGATE METHYLOTROPH; METHYLOPHILUS-METHYLOTROPHUS; METHANOL OXIDATION; STRUCTURAL GENE; DEHYDROGENASE; PURIFICATION
Record Number:CaltechAUTHORS:CHIjbact91a
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:CHIjbact91a
Alternative URL:http://www.pubmedcentral.gov/articlerender.fcgi?artid=208325
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:2990
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:10 May 2006
Last Modified:26 Dec 2012 08:52

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