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Active Carboxylic Acid-Terminated Alkanethiol Self-Assembled Monolayers on Gold Bead Electrodes for Immobilization of Cytochromes c

Tanimura, Ryutaro and Hill, Michael G. and Margoliash, Emanuel and Niki, Katsumi and Ohno, Hiroyuki and Gray, Harry B. (2002) Active Carboxylic Acid-Terminated Alkanethiol Self-Assembled Monolayers on Gold Bead Electrodes for Immobilization of Cytochromes c. Electrochemical and Solid-State Letters, 5 (12). E67-E70. ISSN 1099-0062. http://resolver.caltech.edu/CaltechAUTHORS:TANessl02

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Abstract

It is extremely difficult to immobilize cytochrome c (cyt c) on carboxylic acid-terminated alkanethiol self-assembled monolayers (HOOC-SAM) on gold bead electrodes prepared in a hydrogen flame. We found that simple pretreatment of a HOOC-SAM/gold bead electrode by potential cycling in buffer solution in the range ±300 mV prior to immobilization of the protein facilitated stable cyt c binding to HOOC-SAMs. The stability of cyt c on the HOOC-SAMs is independent of the topology of the gold surface.


Item Type:Article
Additional Information:©2002 The Electrochemical Society Manuscript submitted June 12, 2002; revised manuscript received August 23, 2002. Available electronically October 7, 2002. Work at Caltech was supported by NIH, NSF, and the Arnold and Mabel Beckman Foundation. E.M. acknowledges support from the Edward Mallinckrodt, Jr., Foundation. Work at Occidental College was supported by the David and Lucille Packard Foundation’s Initiative for Interdisciplinary Research. The authors thank Dojindo Molecular Technology for providing carboxylic acid-terminated alkanethiols. California Institute of Technology assisted in meeting the publication costs of this article.
Subject Keywords:proteins; molecular biophysics; organic compounds; self-assembly; monolayers; gold; electrochemistry
Record Number:CaltechAUTHORS:TANessl02
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:TANessl02
Alternative URL:http://dx.doi.org/10.1149/1.1517770
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:2998
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:10 May 2006
Last Modified:26 Dec 2012 08:52

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