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Vital dye labelling demonstrates a sacral neural crest contribution to the enteric nervous system of chick and mouse embryos

Serbedzija, George N. and Burgan, Scott and Fraser, Scott E. and Bronner, Marianne E. (1991) Vital dye labelling demonstrates a sacral neural crest contribution to the enteric nervous system of chick and mouse embryos. Development, 111 (4). pp. 857-866. ISSN 0950-1991. http://resolver.caltech.edu/CaltechAUTHORS:20120417-095608741

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Abstract

We have used the vital dye, DiI, to analyze the contribution of sacral neural crest cells to the enteric nervous system in chick and mouse embryos. In order to label premigratory sacral neural crest cells selectively, DiI was injected into the lumen of the neural tube at the level of the hindlimb. In chick embryos, DiI injections made prior to stage 19 resulted in labelled cells in the gut, which had emerged from the neural tube adjacent to somites 29–37. In mouse embryos, neural crest cells emigrated from the sacral neural tube between E9 and E9.5. In both chick and mouse embryos, DiI-labelled cells were observed in the rostral half of the somitic sclerotome, around the dorsal aorta, in the mesentery surrounding the gut, as well as within the epithelium of the gut. Mouse embryos, however, contained consistently fewer labelled cells than chick embryos. DiI-labelled cells first were observed in the rostral and dorsal portion of the gut. Paralleling the maturation of the embryo, there was a rostral-to-caudal sequence in which neural crest cells populated the gut at the sacral level. In addition, neural crest cells appeared within the gut in a dorsal-to-ventral sequence, suggesting that the cells entered the gut dorsally and moved progressively ventrally. The present results resolve a long-standing discrepancy in the literature by demonstrating that sacral neural crest cells in both the chick and mouse contribute to the enteric nervous system in the postumbilical gut.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dev.biologists.org/content/111/4/857PublisherUNSPECIFIED
Additional Information:© 1991 The Company of Biologists Limited. Accepted 15 January 1991.
Subject Keywords:cell migration, gut, Dil, chick, mouse, enteric nervous system, neural crest
Record Number:CaltechAUTHORS:20120417-095608741
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20120417-095608741
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:30128
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:17 Apr 2012 17:51
Last Modified:26 Dec 2012 15:04

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