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Silencing of the Expression of the Immunoglobulin Kappa Gene in Non-B Cells

Pierce, Jacqueline W. and Gifford, Ann M. and Baltimore, David (1991) Silencing of the Expression of the Immunoglobulin Kappa Gene in Non-B Cells. Molecular and Cellular Biology, 11 (3). pp. 1431-1437. ISSN 0270-7306. http://resolver.caltech.edu/CaltechAUTHORS:20120419-081351781

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Abstract

Although the activating factor NF-_K B can be present in the nucleus of many cell types, transcription and rearrangement of the immunoglobulin kappa chain gene is restricted to cells of the B lineage. Part of this specificity is determined by sequences within the major intron of the kappa gene that specifically silence gene expression in non-B cells (T cells and HeLa cells). These sequences are found in a 232-bp fragment located 5' of the NF-K B binding sequence of the enhancer. When this fragment is added back upstream of an active NF-_K B site, it specifically decreases the expression of a linked gene by more than 10-fold in activated T cells but it has no effect on expression in B cells. The kappa silencer region acts in an orientation- and distance-independent manner and appears to be composed of multiple negative elements. The kappa silencer may act to restrict transcription and rearrangement of the C_K locus to cells of the B lineage.


Item Type:Article
Related URLs:
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http://mcb.asm.org/content/11/3/1431.abstractPublisherUNSPECIFIED
Additional Information:© 1991 American Society for Microbiology. Received 9 October 1990. Accepted 10 December 1990. We thank Astar Winoto, Mark S. Schlissel, and Ranjan Sen for helpful comments on the manuscript. This work was funded by a Special Fellowship of the Leukemia Society of America to J.W.P., Public Health Service grant GM-39458 to D.B., and Public Health Service grant GM-43874-01 from the National Institutes of Health to Ranjan Sen.
Funders:
Funding AgencyGrant Number
Leukemia Society of America Special FellowshipUNSPECIFIED
Public Health ServiceGM-39458
NIH GM-43874-01
Record Number:CaltechAUTHORS:20120419-081351781
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20120419-081351781
Official Citation:Silencing of the expression of the immunoglobulin kappa gene in non-B cells. J W Pierce, A M Gifford, and D Baltimore Mol. Cell. Biol. March 1991 11:1431-1437; doi:10.1128/MCB.11.3.1431
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:30191
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:19 Apr 2012 18:12
Last Modified:26 Dec 2012 15:05

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