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Collagens in avian neural crest development: distribution in vivo and migration-promoting ability in vitro

Perris, Roberto and Krotoski, Danuta and Bronner, Marianne E. (1991) Collagens in avian neural crest development: distribution in vivo and migration-promoting ability in vitro. Development, 113 (3). pp. 969-984. ISSN 0950-1991. http://resolver.caltech.edu/CaltechAUTHORS:20120419-110343819

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Abstract

This study examines the spatiotemporal distribution of collagen (Col) types I-V and IX during neural crest development in vivo and their ability to support neural crest cell movement in vitro. Col I, III and IV were widespread throughout the embryo, including the neural crest migratory pathways, whereas Col II, V and IX preferentially localized to regions from which migrating neural crest cells were absent. Col I-IV and IX occurred both in association with basement membranes and within interstitial matrices, whereas Col V only was detected in juxtaposition to basement membranes. Although initially distributed throughout the rostrocaudal extent of the somitic sclerotome, Col I and III rearranged to the caudal portion with progressive neural crest cell migration through the rostral portion of the sclerotome. This rearrangement does not occur in neural crest-ablated embryos, suggesting that it is a direct consequence of neural crest cell migration. The perinotochordal matrix, avoided by neural crest cells, contained a metameric Col II/IX immunoreactivity along the rostrocaudal axis which alternated with that of Col I and III. In contrast, Col IV and V were not observed in this matrix, but lined the basement membranes of the notochord and ventrolateral neural tube. To determine their functional significance for neural crest cell migration in vivo, purified collagens were tested for their ability to promote neural crest cell motility in vitro. Neural crest cell migration on isolated collagens was most pronounced on Col I and IV, whereas Col II, V and the triple-helical fragment of Col VII were unable to support cell motility. Substrata created by copolymerization of Col I and fibronectin, or Col I and laminin-nidogen, supported cell motility better than Col I alone, whereas both Col V and a cartilage-type chondroitin sulfate proteoglycan reduced cell movement on Col I. Fibronectin bound to pre-immobilized monomeric Col I, II or V had a reduced ability to support neural crest cell movement when compared to fibronectin alone. A similar reduction was seen for Col IV bound to the low density heparan sulfate proteoglycan from the EHS mouse tumor. The results demonstrate that Col I-IX are differentially distributed in the early avian embryo. During neural crest development several of these collagens undergo dynamic reorganizations that correlate with the migration of neural crest cells. Furthermore, various collagens possess distinct abilities to support neural crest cell migration in vitro, and their migration-promoting activity can be modulated by their conformation and/or association with other matrix components.


Item Type:Article
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http://dev.biologists.org/content/113/3/969.abstractPublisherUNSPECIFIED
Additional Information:© 1991 Company of Biologists Limited. Accepted 12 August 1991. We are grateful to Drs Peter Bruckner, Staffan Johansson, Charles Little, Mats Paulsson, Helene Sage, and Jorgen Wieslander for providing various antibodies, purified collagens and other matrix components. Drs Robert Burgeson, Thomas Lallier and Helene Sage are thanked for their valuable suggestions and critical reading of the manuscript. Michael Artinger, Kristin Artinger, Mary Flowers and Susan Tran are acknowledged for their excellent technical assistance. The work was supported by NIH grant USPHS HD-15527 and by the Muscular Dystrophy Association.
Funders:
Funding AgencyGrant Number
NIH USPHSHD-15527
Muscular Dystrophy AssociationUNSPECIFIED
Subject Keywords:collagens, neural crest, avian embryo, cell migration.
Record Number:CaltechAUTHORS:20120419-110343819
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20120419-110343819
Official Citation: R. Perris, D. Krotoski, and M. Bronner-Fraser Collagens in avian neural crest development: distribution in vivo and migration-promoting ability in vitro Development 1991 113:969-984
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:30199
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:19 Apr 2012 18:16
Last Modified:26 Dec 2012 15:05

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