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Purification of DNA polymerase II stimulatory factor I, a yeast single-stranded DNA-binding protein

Brown, William C. and Smiley, Jean K. and Campbell, Judith L. (1990) Purification of DNA polymerase II stimulatory factor I, a yeast single-stranded DNA-binding protein. Proceedings of the National Academy of Sciences of the United States of America, 87 (2). pp. 677-681. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:20120424-083224717

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Abstract

Incidental to the purification of yeast DNA polymerase II was the observation that various chromatographic fractions contained activities that stimulated synthesis by this polymerase. In this paper we report the purification and initial characterization of one such factor, stimulatory factor I (SFI). SFI, which is associated with an apparent complex of three polypeptides of 66, 37, and 13.5 kDa, binds preferentially to single-stranded DNA, possibly explaining its ability to stimulate DNA polymerase II. Single-stranded DNA-binding activity is associated with the 66-kDa polypeptide.


Item Type:Article
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URLURL TypeDescription
http://www.pnas.org/content/87/2/677PublisherUNSPECIFIED
Additional Information:© 1990 National Academy of Sciences. Communicated by Norman Davidson, October 24, 1989 (received for review August 30, 1989). This work was supported by grants from the National Institutes of Health (GM25508) and the American Cancer Society (NP-623G). The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
NIHGM25508
American Cancer SocietyNP-623G
Record Number:CaltechAUTHORS:20120424-083224717
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20120424-083224717
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:30270
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:01 May 2012 22:58
Last Modified:26 Dec 2012 15:06

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