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Pathways of trunk neural crest cell migration in the mouse embryo as revealed by vital dye labelling

Serbedzija, George N. and Fraser, Scott E. and Bronner, Marianne E. (1990) Pathways of trunk neural crest cell migration in the mouse embryo as revealed by vital dye labelling. Development, 108 (4). pp. 605-612. ISSN 0950-1991. http://resolver.caltech.edu/CaltechAUTHORS:20120424-102930429

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Abstract

Analysis of neural crest cell migration in the mouse has been difficult due to the lack of reliable cell markers. Recently, we found that injection of DiI into the chick neural tube marks premigratory neural crest cells whose endfeet are in contact with the lumen of the neural tube (Serbedzija et al. Development 106, 809–819 (1989)). In the present study, this technique was applied to study neural crest cell migratory pathways in the trunk of the mouse embryo. Embryos were removed from the mother between the 8th and the 10th days of development and DiI was injected into the lumen of the neural tube. The embryos were then cultured for 12 to 24 h, and analyzed at the level of the forelimb. We observed two predominant pathways of neural crest cell migration: (1) a ventral pathway through the rostral portion of the somite and (2) a dorsolateral pathway between the dermamyotome and the epidermis. Neural crest cells were observed along the dorsolateral pathway throughout the period of migration. The distribution of labelled cells along the ventral pathway suggested that there were two overlapping phases of migration. An early ventrolateral phase began before E9 and ended by E9.5; this pathway consisted of a stream of cells within the rostral sclerotome, adjacent to the dermamyotome, that extended ventrally to the region of the sympathetic ganglia and the dorsal aorta.


Item Type:Article
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http://dev.biologists.org/content/108/4/605PublisherUNSPECIFIED
Additional Information:© 1990 The Company of Biologists Limited. Accepted 5 January 1990. We thank Scott Burgan for excellent technical assistance and Mark Cooper for suggesting the use of the sucrose solution. This work was supported by USPHS Grant HD-25138 and the March of Dimes Birth Defects Foundation.
Funders:
Funding AgencyGrant Number
U. S. Public Health Service (USPHS)HD-25138
March of Dimes Birth Defects FoundationUNSPECIFIED
Record Number:CaltechAUTHORS:20120424-102930429
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20120424-102930429
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:30275
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:01 May 2012 21:01
Last Modified:26 Dec 2012 15:06

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