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Identification of proteins in the postsynaptic density fraction by mass spectrometry

Walikonis, Randall S. and Jensen, Ole N. and Mann, Matthias and Provance, D. William, Jr. and Mercer, John A. and Kennedy, Mary B. (2000) Identification of proteins in the postsynaptic density fraction by mass spectrometry. Journal of Neuroscience, 20 (11). pp. 4069-4080. ISSN 0270-6474. http://resolver.caltech.edu/CaltechAUTHORS:20120424-150408608

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Abstract

Our understanding of the organization of postsynaptic signaling systems at excitatory synapses has been aided by the identification of proteins in the postsynaptic density (PSD) fraction, a subcellular fraction enriched in structures with the morphology of PSDs. In this study, we have completed the identification of most major proteins in the PSD fraction with the use of an analytical method based on mass spectrometry coupled with searching of the protein sequence databases. At least one protein in each of 26 prominent protein bands from the PSD fraction has now been identified. We found 7 proteins not previously known to be constituents of the PSD fraction and 24 that had previously been associated with the PSD by other methods. The newly identified proteins include the heavy chain of myosin-Va (dilute myosin), a motor protein thought to be involved in vesicle trafficking, and the mammalian homolog of the yeast septin protein cdc10, which is important for bud formation in yeast. Both myosin-Va and cdc10 are threefold to fivefold enriched in the PSD fraction over brain homogenates. Immunocytochemical localization of myosin-Va in cultured hippocampal neurons shows that it partially colocalizes with PSD-95 at synapses and is also diffusely localized in cell bodies, dendrites, and axons. Cdc10 has a punctate distribution in cell bodies and dendrites, with some of the puncta colocalizing with PSD-95. The results support a role for myosin-Va in transport of materials into spines and for septins in the formation or maintenance of spines.


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http://www.jneurosci.org/content/20/11/4069PublisherUNSPECIFIED
Additional Information: © 2000 Society for Neuroscience. Received Jan. 28, 2000; revised March 10, 2000; accepted March 17, 2000. This work was supported by National Institutes of Health Grant NS28710 (M.B.K.), by a fellowship from the FRAXA Research Foundation (R.S.W.), by a fellowship from the European Union Biotechnology Program (O.N.J.), and by the European Molecular Biology Laboratory (EMBL) (O.N.J. and M.M.). We thank Anna Shevchenko (EMBL) for expert technical assistance with preparation of protein samples for mass spectrometry and Lisa Evans for advice on early experiments.
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Funding AgencyGrant Number
NIHNS28710
FRAXA Research FoundationUNSPECIFIED
European Union Biotechnology ProgramUNSPECIFIED
European Molecular Biology Laboratory (EMBL)UNSPECIFIED
Subject Keywords:synaptic transmission; myosin; septins; vesicle transport; signal transduction; multiprotein complex
Record Number:CaltechAUTHORS:20120424-150408608
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20120424-150408608
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:30310
Collection:CaltechAUTHORS
Deposited By: Melanie Stefan
Deposited On:25 Apr 2012 22:04
Last Modified:26 Dec 2012 15:07

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