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Heteroduplex analysis of tra delta f' plasmids and the mechanism of their formation

Guyer, Mark S. and Davidson, Norman and Clark, Alvin J. (1977) Heteroduplex analysis of tra delta f' plasmids and the mechanism of their formation. Journal of Bacteriology, 131 (3). pp. 970-980. ISSN 0021-9193. http://resolver.caltech.edu/CaltechAUTHORS:GUYjbact77

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Abstract

Four tra delta FargG+ plasmids, derived from matings between Hfr AB312 and a recA recipient, have been shown to have deletions of at least 50% of the F genome, including the region in which the tra genes map. The mutant plasmids do contain the F genes required for plasmid maintenance. Correlations can be made between, on the one hand, the F genes present on the tradelta F' plasmids and the F genes transferred early by an Hfr donor, and, on the other hand, the F genes deleted from the tradelta F' plasmids and the F genes transferred late by an Hfr donor. A biased representation of proximally and distally transferred chromosomal markers among the tradelta F' elements was also demonstrated. Taken Taken together, the asymmetrical representation of Hfr genes and the cis dominance of the Tra phenotype of these mutants can best be explained by the hypothesis that the tradelta F' plasmids are formed by repliconation of the transferred exogenote in a recA recipient.


Item Type:Article
Additional Information:Copyright © 1977 by the American Society for Microbiology. Received for publication 15 April 1977 We appreciate the help of Barbara De Larco in the preparation of the manuscript. This work was supported by Public Health Service grant AI-05371 to Alvin J. Clark from the National Institute of Allergy and Infectious Diseases and by Public Health Service grant GM 10991 to Norman Davidson from the National Institute of General Medical Sciences. Mark Guyer was a Fellow of the Jane Coffin Childs Memorial Fund for Medical Research. Errata. Heteroduplex Analysis of tra{Delta}F' Plasmids and the Mechanism of Their Formation J. Bacteriol. 1978 133:1051.
Record Number:CaltechAUTHORS:GUYjbact77
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:GUYjbact77
Alternative URL:http://jb.asm.org/cgi/content/abstract/131/3/970
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ID Code:3032
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Deposited On:11 May 2006
Last Modified:26 Dec 2012 08:52

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