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Tetanic Stimulation Leads to Increased Accumulation of Ca^(2+)/Calmodulin-Dependent Protein Kinase II via Dendritic Protein Synthesis in Hippocampal Neurons

Ouyang, Yannan and Rosenstein, Alan and Kreiman, Gabriel and Schuman, Erin M. and Kennedy, Mary B. (1999) Tetanic Stimulation Leads to Increased Accumulation of Ca^(2+)/Calmodulin-Dependent Protein Kinase II via Dendritic Protein Synthesis in Hippocampal Neurons. Journal of Neuroscience, 19 (18). pp. 7823-7833. ISSN 0270-6474. http://resolver.caltech.edu/CaltechAUTHORS:20120424-154215710

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Abstract

mRNA for the ɑ-subunit of CaMKII is abundant in dendrites of neurons in the forebrain (Steward, 1997). Here we show that tetanic stimulation of the Schaffer collateral pathway causes an increase in the concentration of ɑ-CaMKII in the dendrites of postsynaptic neurons. The increase is blocked by anisomycin and is detected by both quantitative immunoblot and semiquantitative immunocytochemistry. The increase in dendritic ɑ-CaMKII can be measured 100-200 µm away from the neuronal cell bodies as early as 5 min after a tetanus. Transport mechanisms for macromolecules from neuronal cell bodies are not fast enough to account for this rapid increase in distal portions of the dendrites. Therefore, we conclude that dendritic protein synthesis must produce a portion of the newly accumulated CaMKII. The increase in concentration of dendritic CaMKII after tetanus, together with the previously demonstrated increase in autophosphorylated CaMKII (Ouyang et al., 1997), will produce a prolonged increase in steady-state kinase activity in the dendrites, potentially influencing mechanisms of synaptic plasticity that are controlled through phosphorylation by CaMKII.


Item Type:Article
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http://www.jneurosci.org/content/19/18/7823.abstractPublisherArticle
Additional Information:© 1999 Society for Neuroscience. Received March 10, 1999; revised July 1, 1999; accepted July 2, 1999. This work was supported by National Institutes of Health Research Service Award NS10660 (Y.O.), Grants MH49176 and NS17660 (M.B.K.) and NS32792 (E.M.S.), and grants from the Alfred P. Sloan Foundation, Beckman Foundation, John Merck Fund, and PEW Charitable Trusts (E.M.S.). We thank Dr. Scott Fraser, director of the Caltech Biological Imaging Resource Center, for valuable technical advice and for use of the confocal microscope.
Group:Koch Laboratory, KLAB
Funders:
Funding AgencyGrant Number
NIH Research Service AwardNS10660
NIHMH49176
NIHNS17660
NIHNS32792
Alfred P. Sloan FoundationUNSPECIFIED
Beckman FoundationUNSPECIFIED
John Merck FundUNSPECIFIED
Pew Charitable TrustUNSPECIFIED
Subject Keywords:long-term potentiation; protein phosphorylation; synapse; synaptic regulation; synaptic plasticity; immunocytochemistry; hippocampal slices
Record Number:CaltechAUTHORS:20120424-154215710
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20120424-154215710
Official Citation:Yannan Ouyang, Alan Rosenstein, Gabriel Kreiman, Erin M. Schuman, and Mary B. Kennedy Tetanic Stimulation Leads to Increased Accumulation of Ca2+/Calmodulin-Dependent Protein Kinase II via Dendritic Protein Synthesis in Hippocampal Neurons The Journal of Neuroscience, 15 September 1999, 19(18):7823-7833
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:30321
Collection:CaltechAUTHORS
Deposited By: Melanie Stefan
Deposited On:26 Apr 2012 14:34
Last Modified:03 Apr 2014 20:48

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