Cho, Kyung-Ok and Wall, John B. and Pugh, Phyllis C. and Ito, Masamichi and Mueller, Shelley A. and Kennedy, Mary B. (1991) The ɑ subunit of type II Ca^(2+)/calmodulin-dependent protein kinase is highly conserved in Drosophila. Neuron, 7 (3). pp. 439-450. ISSN 0896-6273 http://resolver.caltech.edu/CaltechAUTHORS:20120424-154229132
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A monoclonal antibody against rat brain type II Ca^(2+)/calmodulin-dependent protein kinase (CaM kinase) precipitates three proteins from Drosophila heads with apparent molecular weights similar to those of the subunits of the rat brain kinase. Fly heads also contain a CaM kinase activity that becomes partially independent of Ca^(2+) after autophosphorylation, as does the rat brain kinase. We have isolated a Drosophila cDNA encoding an amino acid sequence that is 77% identical to the sequence of the rat alpha subunit. All known autophosphorylation sites are conserved, including the site that controls Ca^(2+)-independent activity. The gene encoding the cDNA is located between 102E and F on the fourth chromosome. The protein product of this gene is expressed at much higher levels in the fly head than in the body. Thus, both the amino acid sequence and the tissue specificity of the mammalian kinase are highly conserved in Drosophila.
|Alternate Title:||Alpha subunit of type II Ca2+/calmodulin-dependent protein kinase is highly conserved in Drosophila|
|Additional Information:||© 1991 by Cell Press. Received April 11, 1991; revised May 28, 1991. Available online 20 April 2004. We thank Kwang-Wook Choi for Figures 68 and 6C and Debbie Leonard for initial experiments in this project. We also thank Kai Zinn, Bruce Patton, Show-Ling Shyng, and Kwang-Wook Choi for comments on the manuscript. This investigation was supported by National Science Foundation grant BNS-8810418, a National Institutes of Health Biomedical Research Support Grant, the Gustavus and Louise Pfeiffer Research Foundation, the Joseph W. Drown Foundation, a National Research Service Award to M. I., and S. U. R. F. fellowships to J. B. W. and P. C. P. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 USC Section 1734 solely to indicate this fact.|
|Official Citation:||Kyung-Ok Cho, John B. Wall, Phyllis C. Pugh, Masamichi Ito, Shelley A. Mueller, Mary B. Kennedy, The α subunit of type II Ca2+/calmodulin-dependent protein kinase is highly conserved in Drosophila, Neuron, Volume 7, Issue 3, September 1991, Pages 439-450, ISSN 0896-6273, 10.1016/0896-6273(91)90296-C. (http://www.sciencedirect.com/science/article/pii/089662739190296C)|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Melanie Stefan|
|Deposited On:||25 Apr 2012 17:53|
|Last Modified:||25 Apr 2012 18:39|
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