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Lysophosphatidyl choline facilitates labeling of CNS projections with horseradish peroxidase

Frank, Eric and Harris, William A. and Kennedy, Mary B. (1980) Lysophosphatidyl choline facilitates labeling of CNS projections with horseradish peroxidase. Journal of Neuroscience Methods, 2 (2). pp. 183-189. ISSN 0165-0270.

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Previously, it has been difficult to label sensory terminations in the CNS by back-filling peripheral sensory nerves with horseradish peroxidase (HRP), apparently because the amount of HRP transported along the axons is insufficient. Labeling of central sensory projections in axolotls and frogs can be improved by agents known to disrupt membranes. Of those tested, L-alpha-lysophosphatidyl choline is the most effective. The addition of this detergent ot the HRP solution dramatically increases both the number of axons labeled and the density of label within single axons.

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Kennedy, Mary B.0000-0003-1369-0525
Additional Information:Copyright © 1980 Published by Elsevier B.V. Received 27 September 1979. Revised 15 November 1979. Accepted 2 December 1979. We thanks Dr. S. LeVay for suggesting the use of lysolecithin. This work was supported by the Milton Fund and NIH Grants EY 00605 and NS 14451.
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NIHEY 00605
NIHNS 14451
Record Number:CaltechAUTHORS:20120424-154252993
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:30353
Deposited By: Melanie Stefan
Deposited On:25 Apr 2012 17:00
Last Modified:10 Apr 2015 17:23

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