Guyer, Mark S. and Figurski, David and Davidson, Norman (1976) Electron microscope study of a plasmid chimera containing the replication region of the Escherichia coli F plasmid. Journal of Bacteriology, 127 (2). pp. 988-997. ISSN 0021-9193 http://resolver.caltech.edu/CaltechAUTHORS:GUYjbact76
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pML31, a plasmid chimera constructed to contain the replication genes of an Flac plasmid, has been studied by electron microscope methods. Heteroduplex analysis shows that the only F sequence present in pML31 is that with corrdinates 40.3-49.3F. This region has previously been identified as essential for plasmid maintenance. The sequence of pML31, which was derived originally from R6-5, carries the km gene(s) and an inverted duplication of a 1.0-kilobase sequence. On the basis of length measurements, the repeated sequence is different from IS1, IS2, IS3, and an inverted repeat associated with the km gene(s) of plasmid JR67.
|Additional Information:||Copyright © 1976 by the American Society for Microbiology. Received for publication 3 February 1976 This research has been supported by Public Health Service grants GM10991 from the National Institute of General Medical Sciences and AI-07194 from the National Institute of Allergy and Infectious Diseases, and National Science Foundation grant GB-29492. Mark Guyer is a Fellow of the Jane Coffin Childs Memorial Fund for Medical Research. David Figurski is a Public Health Service research fellow (1-F22-AI-01412, National Institute of Allergy and Infectious Diseases).|
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|Deposited On:||11 May 2006|
|Last Modified:||26 Dec 2012 08:52|
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