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Electron microscope study of a plasmid chimera containing the replication region of the Escherichia coli F plasmid

Guyer, Mark S. and Figurski, David and Davidson, Norman (1976) Electron microscope study of a plasmid chimera containing the replication region of the Escherichia coli F plasmid. Journal of Bacteriology, 127 (2). pp. 988-997. ISSN 0021-9193. http://resolver.caltech.edu/CaltechAUTHORS:GUYjbact76

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Abstract

pML31, a plasmid chimera constructed to contain the replication genes of an Flac plasmid, has been studied by electron microscope methods. Heteroduplex analysis shows that the only F sequence present in pML31 is that with corrdinates 40.3-49.3F. This region has previously been identified as essential for plasmid maintenance. The sequence of pML31, which was derived originally from R6-5, carries the km gene(s) and an inverted duplication of a 1.0-kilobase sequence. On the basis of length measurements, the repeated sequence is different from IS1, IS2, IS3, and an inverted repeat associated with the km gene(s) of plasmid JR67.


Item Type:Article
Additional Information:Copyright © 1976 by the American Society for Microbiology. Received for publication 3 February 1976 This research has been supported by Public Health Service grants GM10991 from the National Institute of General Medical Sciences and AI-07194 from the National Institute of Allergy and Infectious Diseases, and National Science Foundation grant GB-29492. Mark Guyer is a Fellow of the Jane Coffin Childs Memorial Fund for Medical Research. David Figurski is a Public Health Service research fellow (1-F22-AI-01412, National Institute of Allergy and Infectious Diseases).
Record Number:CaltechAUTHORS:GUYjbact76
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:GUYjbact76
Alternative URL:http://jb.asm.org/cgi/content/abstract/127/2/988
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:3036
Collection:CaltechAUTHORS
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Deposited On:11 May 2006
Last Modified:26 Dec 2012 08:52

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