Stitt, Barbara L. and Revel, Helen R. and Lielausis, Ilga and Wood, William B. (1980) Role of the host cell in bacteriophage T4 development. II. Characterization of host mutants that have pleiotropic effects on T4 growth. Journal of Virology, 35 (3). pp. 775-789. ISSN 0022-538X http://resolver.caltech.edu/CaltechAUTHORS:STIjvir80
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Mutant host-defective Escherichi coli that fail to propagate bacteriophage T4 and have a pleiotropic effect on T4 development have been isolated and characterized. In phage-infected mutant cells, specific early phage proteins are absent or reduced in amount, phage DNA synthesis is depressed by about 50%, specific structural phage proteins, including some tail and collar components, are deficient or missing, and host-cell lysis is delayed and slow. Almost all phage that can overcome the host block carry mutantions that map in functionally undefined 'nonessential' regions of the T4 genome, most near gene 39. The mutant host strains are temperature sensitive for growth and show simultaneous reversion of the ts phenotype and the inability to propagate T4+. The host mutations are cotransduced with ilv (83 min) and may lie in the gene for transcription termination factor rho.
|Additional Information:||Copyright © 1980 by the American Society for Microbiology. These studies were supported by research grants to W.B.W. from the Public Health Service National Institute of Allergy and Infectious Diseases (AI-09238, AI-14994) and from the American Cancer Society, California Division (Special Grant 573). B.L.S. was also supported by a Public Health Service predoctoral training grant (GM-00086 from the National Institute of General Medical Science) to the California Institute of Technology, a Caltech Special Institute Fellowship, and the Arthur McCallum Fund.|
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|Deposited On:||15 May 2006|
|Last Modified:||26 Dec 2012 08:52|
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