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Process of infection with bacteriophage phi chi 174. XL. Viral DNA replication of phi chi 174 mutants blocked in progeny single-stranded DNA synthesis

Fukuda, Akio and Sinsheimer, Robert L. (1976) Process of infection with bacteriophage phi chi 174. XL. Viral DNA replication of phi chi 174 mutants blocked in progeny single-stranded DNA synthesis. Journal of Virology, 18 (1). pp. 218-226. ISSN 0022-538X. http://resolver.caltech.edu/CaltechAUTHORS:FUKjvir76b

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Abstract

Mutation in several different cistrons of bacteriophage phi chi 174 blocks net progeny single-stranded DNA synthesis at the late period of infection (15). For the study of the functions of these cistrons in single-stranded DNA synthesis, asymmetric replication of replicative form DNA was examined at the late period of infection with amber mutants of these cistrons. While the normal, rapid process of asymmetric single-stranded viral DNA synthesis is blocked at the late period of these mutant infections, an asymmetric synthesis of the viral strand of replicative-form DNA is observed in this period, though at a reduced level, together with degradation of prelabeled viral strand. Some intermediate replicative-form molecules were also detected. Asymmetric synthesis of the viral strand of replicative-form DNA at the late period of phi chi infection is completely inhibited in the presence of a low concentration (35mug/ml) of chloramphenicol (which also blocks net single-stranded viral DNA synthesis). These results are discussed in terms of the possible role of the specific viral proteins for normal single-stranded DNA synthesis.


Item Type:Article
Additional Information:Copyright © 1976 by the American Society for Microbiology. Received for publication 22 September 1975 This work was supported in part by Public Health Service research grant GM-13554 from the National Institute of General Medical Sciences.
Record Number:CaltechAUTHORS:FUKjvir76b
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:FUKjvir76b
Alternative URL:http://jvi.asm.org/cgi/content/abstract/18/1/218
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:3120
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:16 May 2006
Last Modified:26 Dec 2012 08:52

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