CaltechAUTHORS
  A Caltech Library Service

Process of infection with bacteriophage phi X 174 XXXVIII. Replication of phi chi 174 replicative form in vivo

Fukuda, Akio and Sinsheimer, Robert L. (1976) Process of infection with bacteriophage phi X 174 XXXVIII. Replication of phi chi 174 replicative form in vivo. Journal of Virology, 17 (3). pp. 776-787. ISSN 0022-538X. http://resolver.caltech.edu/CaltechAUTHORS:FUKjvir76a

[img]
Preview
PDF
See Usage Policy.

1658Kb

Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:FUKjvir76a

Abstract

The replication of bacteriophage phi X 174 replicative-form DNA has been studied by structural analysis of pulse-labeled replicative-intermediate molecules. Such intermediates were identified by pulse-labeling with [13H]thymidine and separated into four major fractions (A, B, C, and D) in a propidium diiodide-cesium chloride buoyand density gradient. Sedimentation analysis of each of these fractions suggests the following features of phi X replicative-form DNA replication in vivo. (i) At the end of one cycle of replication, one daughter replicative form (RFII) contains a nascent plus (+) strand of the unit viral length, and the other daughter RFII contains small fragments of nascent minus (-) strand. (ii) Asymmetry is also associated with production of the first supercoiled RFI after addition of pulse label in that only the minus strand becomes radioactive. (iii) A supercoiled DNA (RFI') seems to occur in vivo. This DNA is observed at a position of greater density in a propidium diiodide-cesium chloride buoyant density gradient than normal RFI. (iv) A novel DNA component is observed, at a density greater than RFI, which releases, in alkali, a plus strand longer (1.5 to 1.7 times) than the unit viral length. These results are discussed in terms of the possible sequence of events in phi X 174 replicative-form replication in vivo.


Item Type:Article
Additional Information:Copyright © 1976 by the American Society for Microbiology. Received for publication 2 September 1975 This work was supported by Public Health Service grant GM-13554 from the National Institute of General Medical Sciences.
Record Number:CaltechAUTHORS:FUKjvir76a
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:FUKjvir76a
Alternative URL:http://jvi.asm.org/cgi/content/abstract/17/3/776
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:3122
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:16 May 2006
Last Modified:26 Dec 2012 08:52

Repository Staff Only: item control page