Dempsey, William P. and Fraser, Scott E. and Pantazis, Periklis (2012) SHG nanoprobes: Advancing harmonic imaging in biology. Bioessays, 34 (5). pp. 351-360. ISSN 0265-9247 http://resolver.caltech.edu/CaltechAUTHORS:20120511-095637921
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Second harmonic generating (SHG) nanoprobes have recently emerged as versatile and durable labels suitable for in vivo imaging, circumventing many of the inherent drawbacks encountered with classical fluorescent probes. Since their nanocrystalline structure lacks a central point of symmetry, they are capable of generating second harmonic signal under intense illumination – converting two photons into one photon of half the incident wavelength – and can be detected by conventional two-photon microscopy. Because the optical signal of SHG nanoprobes is based on scattering, rather than absorption as in the case of fluorescent probes, they neither bleach nor blink, and the signal does not saturate with increasing illumination intensity. When SHG nanoprobes are used to image live tissue, the SHG signal can be detected with little background signal, and they are physiologically inert, showing excellent long-term photostability. Because of their photophysical properties, SHG nanoprobes provide unique advantages for molecular imaging of living cells and tissues with unmatched sensitivity and temporal resolution.
|Additional Information:||© 2012 Wiley Periodicals, Inc. Article first published online: 6 Mar. 2012. WPD was supported by the Caltech Rosen Scholar Graduate Fellowship. This work was supported by the Bioimaging Center at the California Institute of Technology, and the Swiss National Center of Competence in Research (NCCR) "Nanoscale Science". We apologize to authors whose work was not cited due to space limitations.|
|Subject Keywords:||animal imaging; fluorescent nanodiamonds; fluorescent proteins; nonlinear crystals; quantum dots; SERS nanoparticles; upconverting nanoparticles|
|Official Citation:||Dempsey, W. P., Fraser, S. E. and Pantazis, P. (2012), SHG nanoprobes: Advancing harmonic imaging in biology. Bioessays, 34: 351–360. doi: 10.1002/bies.201100106|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Tony Diaz|
|Deposited On:||11 May 2012 18:46|
|Last Modified:||11 May 2012 18:46|
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