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Slow intermixing of cells during Xenopus embryogenesis contributes to the consistency of the blastomere fate map

Wetts, Richard and Fraser, Scott E. (1989) Slow intermixing of cells during Xenopus embryogenesis contributes to the consistency of the blastomere fate map. Development, 105 (1). pp. 9-15. ISSN 0950-1991. http://resolver.caltech.edu/CaltechAUTHORS:20120517-145508292

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Abstract

The relatively consistent fates of the blastomeres of the frog embryo could result from (i) predetermination of the blastomeres or (ii) reproducible morphogenetic cell movements. In some species, the mixing of the cells during development provides a test between these alternative hypotheses. If blastomeres are predetermined, then random intermixing of the descendants with neighboring cells could not alter their fate. To follow cell mixing during Xenopus development, fluorescent dextran lineage tracers were microinjected into identified blastomeres at the 16-cell stage. The labelled descendants of the injected blastomeres were followed over several stages of embryogenesis. After gastrulation, the labelled descendants formed relatively coherent groups in characteristic regions of the embryo. By larval stages, most of the labelled descendants were still located in characteristic regions. However, coherence was less pronounced and individual descendants were located in many regions of the embryo. Hence, cell mixing is a slow, but progressive, process throughout Xenopus development. This is in sharp contrast to the extensive mixing that occurs during the early development of other vertebrates, such as zebrafish and mice. The slow cell mixing in Xenopus development suggests a simple mechanism for the consistent fates of cleavage-stage blastomeres. The stereotyped cell movements of embryogenesis redistribute the largely coherent descendants to characteristic locations in the embryo. The small amount of mixing that does occur would result in variable locations of a small proportion of the descendants; this could contribute to the observed variability of the blastomere fate map. Because cell mixing during Xenopus development is insufficient to challenge possible lineage restrictions, additional experiments must be performed to establish when and if lineage restrictions occur.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dev.biologists.org/content/105/1/9.abstractPublisherUNSPECIFIED
Additional Information:© 1989 The Company of Biologists Limited. Accepted 30 September 1988. This work was supported by grants from the National Science Foundation (BNS 8608356) and the Monsanto Corporation and by a McKnight Foundation Scholar Award (S. E. F.). We thank M. S. Carhart, S. Burgan and S. Olson for their technical assistance. Preliminary results from this work have been previously published (Wetts & Fraser, 1986; Wetts et al. 1988).
Funders:
Funding AgencyGrant Number
NSFBNS 8608356
Monsanto CorporationUNSPECIFIED
McKnight Foundation Scholar Award UNSPECIFIED
Subject Keywords:cell mixing; cell lineage; fate mapping; amphibian gastrulation; fluorescent dextran
Record Number:CaltechAUTHORS:20120517-145508292
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20120517-145508292
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:31534
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:18 May 2012 15:35
Last Modified:26 Dec 2012 15:13

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