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Comparison of exon 5 sequences from 35 class I genes of the BALB/c mouse

Brorson, Kurt A. and Hunt, Stephen W., III and Hunkapiller, Tim and Sun, Y. Henry and Cheroutre, Hilde and Nickerson, Deborah A. and Hood, Leroy (1989) Comparison of exon 5 sequences from 35 class I genes of the BALB/c mouse. Journal of Experimental Medicine, 170 (6). pp. 1837-1858. ISSN 0022-1007. http://resolver.caltech.edu/CaltechAUTHORS:20120531-092327545

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Abstract

DNA sequences of the fifth exon, which encodes the transmembrane domain, were determined for the BALB/c mouse class I MHC genes and used to study the relationships between them. Based on nucleotide sequence similarity, the exon 5 sequences can be divided into seven groups. Although most members within each group are at least 80% similar to each other, comparison between groups reveals that the groups share little similarity. However, in spite of the extensive variation of the fifth exon sequences, analysis of their predicted amino acid translations reveals that only four class I gene fifth exons have frameshifts or stop codons that terminate their translation and prevent them from encoding a domain that is both hydrophobic and long enough to span a lipid bilayer. Exactly 27 of the remaining fifth exons could encode a domain that is similar to those of the transplantation antigens in that it consists of a proline-rich connecting peptide, a transmembrane segment, and a cytoplasmic portion with membrane-anchoring basic residues. The conservation of this motif in the majority of the fifth exon translations in spite of extensive variation suggests that selective pressure exists for these exons to maintain their ability to encode a functional transmembrane domain, raising the possibility that many of the nonclassical class I genes encode functionally important products.


Item Type:Article
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http://dx.doi.org/10.1084/jem.170.6.1837DOIUNSPECIFIED
http://jem.rupress.org/content/170/6/1837PublisherUNSPECIFIED
Additional Information:© 1989 Rockefeller University Press. Received for publication 19 June 1989 and in revised form 22 August 1989. This work was supported by National Institute of Health Grant AI-17565. S. W. Hunt is a Special Fellow of the Leukemia Society of America. D. Nickerson was a visiting associate from the University of South Florida, Tampa, FL. S. W. Hunt's present address is the Division of Rheumatology and Immunology, The University of North Carolina, Chapel Hill, NC 27599. Y. H. Sun's present address is the Institute of Molecular Biology, Academia Sinica, Nankang, Taipei 11529, Taiwan, Republic of China. H. Cheroutre's present address is the Department of Microbiology and Immunology, University of California, Los Angeles, CA 90024. We thank Drs. I. Stroynowski, M. Zuniga, K. Fischer Lindahl, and J. Kobori for critically reviewing this manuscript; Dr. J. Howard for critical insights on exon 5 evolution and sharing unpublished rat exon 5 sequence data; and Mrs. C. Blagg for expert secretarial assistance.
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NIHAI-17565
Record Number:CaltechAUTHORS:20120531-092327545
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20120531-092327545
Official Citation:Comparison of exon 5 sequences from 35 class I genes of the BALB/c mouse. K A Brorson, S W Hunt, 3rd, T Hunkapiller, Y H Sun, H Cheroutre, D A Nickerson, and L Hood 170:1837-1858. doi:10.1084/jem.170.6.1837
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:31735
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:31 May 2012 16:47
Last Modified:26 Dec 2012 15:17

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