Kumar, Vipin and Urban, James L. and Hood, Leroy (1989) In individual T cells one productive α rearrangement does not appear to block rearrangement at the second allele. Journal of Experimental Medicine, 170 (6). pp. 2183-2188. ISSN 0022-1007 http://resolver.caltech.edu/CaltechAUTHORS:20120531-095902090
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The T cell lymphoma line BW5147 has rearranged TCR alpha chain genes segments on both the homologous chromosomes: one is functional (V alpha 1) and the second (V alpha 16.1) is a pseudogene. The extreme 3' position of the V alpha 16.1 gene segment in the V alpha locus allows us to recognize rearrangements of most V alpha gene segments using the V alpha 16 probe as a marker. The absence of the genomic V alpha 16.1 gene fragment in mature thymocytes, antigen-specific T cells, and in more than two-thirds of the peripheral T cells suggests that most T lymphocytes rearrange both alpha loci. It appears that productive alpha chain rearrangement on one allele probably does not block a subsequent rearrangement on the other alpha locus.
|Additional Information:||© 1989 Rockefeller University Press. Received for publication 21 August 1989 and in revised form 15 September 1989. V. Kumar is a postdoctoral fellow of the National Multiple Sclerosis Society and J. L. Urban is supported by a Leukemia Society of American Fellowship. We thank Joan Kobori, Joan Goverman, Ulf Landegren, and Tim Hunkapiller for a critical reading of the manuscript, and Cathy Blagg and Bertha jones for help in preparing the manuscript.|
|Official Citation:||In individual T cells one productive alpha rearrangement does not appear to block rearrangement at the second allele. V Kumar, J L Urban, and L Hood 170:2183-2188. doi:10.1084/jem.170.6.2183|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Ruth Sustaita|
|Deposited On:||31 May 2012 17:56|
|Last Modified:||26 Dec 2012 15:17|
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