Sen, Ranjan and Baltimore, David (1987) In vitro transcription of immunoglobulin genes in a B-cell extract: effects of enhancer and promoter sequences. Molecular and Cellular Biology, 7 (5). pp. 1989-1994. ISSN 0270-7306. PMCID PMC365307. http://resolver.caltech.edu/CaltechAUTHORS:20120626-093550673
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Transfection experiments have led to the identification of three DNA sequences that are responsible for the tissue-specific expression of immunoglobulin genes. As a first step toward characterizing these regulatory phenomena at the biochemical level, we report the development of an in vitro transcription system from cells of the B lymphoid lineage. In these extracts, transcription of the MOPC41 kappa promoter is correctly initiated and dependent on the presence of an upstream sequence element located between -44 and -79 base pairs from the cap site. Second, although standard in vitro transcriptions are not affected by the presence or absence of enhancer sequences, we observed that the addition of polyethylene glycol led to a B-cell extract-specific suppression of transcription from a template that carries an immunoglobulin enhancer.
|Additional Information:||© 1987 American Society for Microbiology. Received 27 May 1986; Accepted 7 January 1987. We thank Andrew Fire, Mark Samuels, and Phillip Sharp for their enthusiasm and help in initiating this project and for generously providing us with major late promoter-containing recombinant plasmids and HeLa whole-cell extracts, David Weaver for critical reading of the manuscript, and Ginger Pierce for quick and efficient typing of the manuscript. Deletions 5'Δ5 and 5'Δ7 were a gift from Yehudit Bergman. This work was supported by a grant from the American Cancer Society to D.B. R.S. is a fellow of the Damon Runyon-Walter Winchell Cancer Fund.|
|PubMed Central ID:||PMC365307|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Jason Perez|
|Deposited On:||26 Jun 2012 19:27|
|Last Modified:||04 Apr 2017 23:14|
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