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Nucleotide sequence of ORF2: an open reading frame upstream of the tRNA ligase gene

Komatsoulis, George A. and Westaway, Shawn K. and Abelson, John N. (1987) Nucleotide sequence of ORF2: an open reading frame upstream of the tRNA ligase gene. Nucleic Acids Research, 15 (21). p. 9079. ISSN 0305-1048. http://resolver.caltech.edu/CaltechAUTHORS:20120626-094010258

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Abstract

During the course of sequencing the tRNA ligase gene from the yeast Saccharomyces cerevisiae , an open reading frame, ORF2, was discovered in its vicinity (1). ORF2 was found to be the locus of a single copy, essential gene (S. Westaway and J. Abelson, unpublished observations) with a transcript starting only 125 nucleotides upstream of tRNA ligase (1). The proximity of ORF2 to tRNA ligase suggested that their products might be transcriptionally coregulated and that its product might participate in the processing of tRNA precursors. For this reason we completely sequenced both strands of the ORF2 DNA using the method of Sanger, et. al. (2) .The sequence is shown below, with the corresponding amino acid sequence of the putative protein product of 623 amino acids (mw 71,300). The left arrows show the transcription starts of tRNA ligase, and the right arrows show the initiation sites of ORF2 transcription. The protein is not significantly homologous to any protein on the NBRF database, leaving its function in question.


Item Type:Article
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http://dx.doi.org/10.1093/nar/15.21.9079DOIUNSPECIFIED
http://nar.oxfordjournals.org/content/15/21/9079.full.pdf+htmlPublisherUNSPECIFIED
Additional Information:© 1987 Oxford University Press. Submitted October 1, 1987. Computer resources were provided by BIONET(TM) National Computer Resource for Molecular Biology, whose funding is provided by the Biomedical Research Technology Program, Division of Research Resources, NIH (grant 1-U41RR-01685-05). This work was supported by a National Research Service Award (5 T32 GM07616-09) from the National Institute of General Medical Sciences to G.A.K. and S.K.W.
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NIH1-U41RR-01685-05
National Institute of General Medical Sciences National Research Award5 T32 GM07616-09
Record Number:CaltechAUTHORS:20120626-094010258
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20120626-094010258
Official Citation: George A. Komatsoulis, Shawn K. Westaway, and John N. Abelson Nucleotide sequence of ORF2: an open reading frame upstream of the tRNA ligase gene Nucl. Acids Res. (1987) 15(21): 9079 doi:10.1093/nar/15.21.9079
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ID Code:32087
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:26 Jun 2012 19:10
Last Modified:26 Jun 2012 19:10

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