Guillory, Georgia and Bronner, Marianne E. (1986) An in vitro assay for neural crest cell migration through the somites. Journal of Embryology and Experimental Morphology, 98 (1). pp. 85-97. ISSN 0022-0752 http://resolver.caltech.edu/CaltechAUTHORS:20120627-082546827
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Neural crest cells in the trunk of the avian embryo come into contact with the somites and neural tube during the course of their migration. However, the relationship between the somites and the early migratory routes followed by these cells is not yet completely understood. Here, we use a tissue culture assay to examine if avian neural crest cells migrate through the somites. Cultures of quail somites were prepared from four adjacent regions along the neural axis in the trunk. Each region had four pairs of consecutive somites with region I being most anterior and region IV containing the last four segments. Within each region, the somites were separated from other tissues by enzymatic digestion and plated onto collagen-coated dishes. Immuno-cytochemical techniques were used to confirm that no neural crest cells, recognized by the HNK-1 antibody, were present on the surface of the somites at the time of explantation. After several days in culture, the explanted somites were screened to identify pigment cells. Because neural crest cells give rise to all of the melanocytes in the trunk, the presence of pigment cells indicated that neural crest precursors were contained within the initial explant. After 5–11 days in vitro, the percentage of somite cultures containing pigment cells in regions I through IV, respectively, was 36%, 51%, 31% and 1%. These results suggest that neural crest cells migrate through the somitic mesenchyme and first enter the somites between 5 to 9 segments rostral to the most recently formed somite.
|Additional Information:||© 1986 The Company of Biologists Limited. Accepted April 22, 1986. We would like to thank Dr Scott Fraser for his helpful comments on the manuscript. This work was supported by USPHS Grant HD-15527-01 and Basic Research Grant 1-896 from the March of Dimes.|
|Subject Keywords:||neural crest, migration, somites, pigment cells, in vitro, quail, Coturnix coturnix japonica.|
|Official Citation:||Georgia Guillory and Marianne Bronner-Fraser An in vitro assay for neural crest cell migration through the somites J Embryol Exp Morphol November 1986 98:85-97|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Ruth Sustaita|
|Deposited On:||27 Jun 2012 15:43|
|Last Modified:||26 Dec 2012 15:24|
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