Robins, Diane M. and Malissen, Marie and Hood, Leroy and Ferreira, Arturo and Walthall, Deborah and Mitchell, Mark (1986) Multiple C4/Slp genes distinguished by expression after transfection. Molecular and Cellular Biology, 6 (1). pp. 134-141. ISSN 0270-7306. http://resolver.caltech.edu/CaltechAUTHORS:20120627-095204785
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The S region of the murine major histocompatibility complex contains two closely related genes: C4, encoding the fourth component of complement, and Slp, encoding sex-limited protein. We cloned these genes from a cosmid library of the B10.W7R strain that does not show androgen regulation of the Slp protein. Restriction site polymorphisms revealed at least four C4-like genes within the S^(w7) locus, indicating evolutionary amplification of this region. Transfection of these genes into L cells resulted in expression, processing, and secretion of immunologically correct C4 and Slp proteins. At least two different Slp genes and one C4 gene were capable, after transfection, of expressing C4 and Slp indistinguishable from macrophage-derived protein. A third Slp gene exists within this locus whose recombinant cognate did not express in L cells. Thus, the B10.W7R S region includes one C4 gene and at least three Slp-like genes.
|Additional Information:||© 1986 American Society for Microbiology. Received 26 July 1985; Accepted 10 October 1985. We thank Helen Doyle for technical assistance, Ruth Montague for art work, and Becky Mitchell for preparing the manuscript. Moses Chao, Laurel Eckhardt, Larry Chasin, and Richard Axel provided helpful discussions. We gratefully acknowledge Victor Nussenzweig for giving us the B10.W7R mouse strain and antisera to initiate this work. Support for this investigation was from Public Health Services grant GM31546 (D.M.R.) from the National Institutes of Health. RIA characterization was supported by Public Health Service grant AI13224 (A.F.) from the National Institutes of Health.|
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|Deposited By:||Jason Perez|
|Deposited On:||27 Jun 2012 20:59|
|Last Modified:||23 Aug 2016 10:13|
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