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Assembly of Protein 4.1 during Chicken Erythroid Differentiation

Staufenbiel, Matthias and Lazarides, Elias (1986) Assembly of Protein 4.1 during Chicken Erythroid Differentiation. Journal of Cell Biology, 102 (4). pp. 1157-1163. ISSN 0021-9525. http://resolver.caltech.edu/CaltechAUTHORS:20120627-131406157

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Abstract

Protein 4.1 is a peripheral membrane protein that strengthens the actin-spectrin based membrane skeleton of the red blood cell and also serves to attach this structure to the plasma membrane. In avian erythrocytes it exists as a family of closely related polypeptides that are differentially expressed during erythropoiesis. We have analyzed the synthesis and assembly onto the membrane skeleton of protein 4.1 and in this paper we show that its assembly is extremely rapid and highly efficient since greater than 95% of the molecules synthesized are assembled in less than 1 min. The remaining minor fraction of unassembled protein 4.1 differs kinetically and is either degraded or assembled with slower kinetics. All protein 4.1 variants exhibit a similar kinetic behavior irrespective of the stage of erythroid differentiation. Thus, the amount and the variants ratio of protein 4.1 assembled are determined largely at the transcriptional or at the translational level and not posttranslationally. During erythroid terminal differentiation the molar amounts of protein 4.1 and spectrin assembled change. In postmitotic cells, as compared with proliferative cells, far more protein 4.1 than spectrin is assembled onto the membrane-skeleton. This modulation may permit the assembly of an initially flexible membrane skeleton in mitotic erythroid cells. As cells become postmitotic and undergo the final steps of maturation the membrane skeleton may be gradually stabilized by the assembly of protein 4.1.


Item Type:Article
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http://dx.doi.org/10.1083/jcb.102.4.1157DOIUNSPECIFIED
http://jcb.rupress.org/content/102/4/1157PublisherUNSPECIFIED
Additional Information:© 1986 Rockefeller University Press. Received for publication 25 October 1985, and in revised form 25 November 1985. We thank Shelly Diamond (California Institute of Technology) for performing the centrifugal elutriation. We also thank Dr. John Ngai (California Institute of Technology) for his valuable comments on the manuscript. This work was supported by grants from the National Institutes of Health, National Science Foundation, and Muscular Dystrophy Association. M. Staufenbiel was also supported by Cancer Research Campaign International Fellowship awarded by the International Union Against Cancer and a Senior lnvestigatorship of the American Heart Association, Greater Los Angeles Affiliate.
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NIHUNSPECIFIED
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Muscular Dystrophy AssociationUNSPECIFIED
Cancer Research Campaign International Fellowship UNSPECIFIED
International Union Against CancerUNSPECIFIED
American Hearth InvestigationUNSPECIFIED
Record Number:CaltechAUTHORS:20120627-131406157
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20120627-131406157
Official Citation:Assembly of protein 4.1 during chicken erythroid differentiation. M Staufenbiel and E Lazarides 102:1157-1163. doi:10.1083/jcb.102.4.1157
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:32146
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:27 Jun 2012 20:57
Last Modified:26 Dec 2012 15:25

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