Conti-Tronconi, Bianca M. and Dunn, Susan M. J. and Barnard, Eric A. and Dolly, J. Oliver and Lai, F. Anthony and Ray, Nandita and Raftery, Michael A. (1985) Brain and muscle nicotinic acetylcholine receptors are different but homologous proteins. Proceedings of the National Academy of Sciences of the United States of America, 82 (15). pp. 5208-5212. ISSN 0027-8424 http://resolver.caltech.edu/CaltechAUTHORS:20120627-145222584
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An α-bungarotoxin-binding protein was purified from chick optic lobe and brain by an improved method. Previous and present observations justify its designation as a brain nicotinic acetylcholine receptor (AcChoR). It contains subunits whose apparent molecular weights are somewhat larger than those of subunits of peripheral AcChoRs. The size of the optic lobe AcChoR complex is greater than that of the peripheral receptor when estimated from its sedimentation behavior. Brain AcChoR subunits can be specifically precipitated by a monoclonal antibody directed against chick muscle AcChoR. Amino-terminal amino acid sequence analysis was performed on AcChoR preparations and isolated subunits from the optic lobe and from the rest of the chick brain. The sequences obtained demonstrate that, at least for the lowest molecular weight component, the AcChoRs from different brain areas are identical and they are highly homologous to muscle AcChoR. It is concluded that the brain alpha-bungarotoxin-binding protein is indeed a nicotinic AcChoR and is encoded by a set of genes that is different from, but strongly related to, that for the muscle AcChoR.
|Additional Information:||© 1985 by the National Academy of Sciences. Communicated by John D. Roberts, March 25, 1985. We thank Dr. Lois D. Hunt of the National Biomedical Research Foundation for analyzing the data and constructing the genealogical tree. We are also grateful to Sylvia Yuen and Lynda Millstine for skillful technical assistance and Phoebe Ray for expert typing. This research was supported by U.S. Public Health Service Grant NS-10294, by U.S. Army Grant ARO DAMD17-82-C-2175, and by the U.K. Medical Research Council (MRC Molecular Neurobiology Research Group, Imperial College). The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.|
|Subject Keywords:||nicotinic receptor sequence analysis; gene families|
|Official Citation:||B M Conti-Tronconi, S M Dunn, E A Barnard, J O Dolly, F A Lai, N Ray, and M A Raftery Brain and muscle nicotinic acetylcholine receptors are different but homologous proteins PNAS 1985 82 (15) 5208-5212|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Ruth Sustaita|
|Deposited On:||27 Jun 2012 23:06|
|Last Modified:||26 Dec 2012 15:25|
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