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Regulatory Region of the Heat Shock-Inducible capR (lon) Gene: DNA and Protein Sequences

Gayda, Randall C. and Stephens, Paul E. and Hewick, Rodney and Schoemaker, Joyce M. and Dreyer, William J. and Markovitz, Alvin (1985) Regulatory Region of the Heat Shock-Inducible capR (lon) Gene: DNA and Protein Sequences. Journal of Bacteriology, 162 (1). pp. 271-275. ISSN 0021-9193. http://resolver.caltech.edu/CaltechAUTHORS:20120628-074326955

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Abstract

The CapR protein is an ATP hydrolysis-dependent protease as well as a DNA-stimulated ATPase and a nucleic acid-binding protein. The sequences of the 5' end of the capR (lon) gene DNA and N-terminal end of the CapR protein were determined. The sequence of DNA that specifies the N-terminal portion of the CapR protein was identified by comparing the amino acid sequence of the CapR protein with the sequence predicted from the DNA. The DNA and protein sequences established that the mature protein is not processed from a precursor form. No sequence corresponding to an SOS box was found in the 5' sequence of DNA. There were sequences that corresponded to a putative -35 and -10 region for RNA polymerase binding. The capR (lon) gene was recently identified as one of 17 heat shock genes in Escherichia coli that are positively regulated by the product of the htpR gene. A comparison of the 5' DNA region of the capR gene with that of several other heat shock genes revealed possible consensus sequences.


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http://jb.asm.org/content/162/1/271.abstractPublisherUNSPECIFIED
Additional Information:© 1985 American Society for Microbiology. Received 22 August 1984. Accepted 4 January 1985. We thank Spencer Emtage for providing DNA sequencing facilities at Celltech Ltd. and Robert Heinrikson and Roger Poorman for protein sequencing facilities at the University of Chicago. We thank Gerald Gordon for assistance with DNA sequencing in Chicago and Marc Charette and Gordon Henderson fot purifying the CapR and CapR9 proteins. We thank Carol A. Gross, Roger Hendrix, Carol Woolford, and Frederick C. Neidhardt for providing the unpublished sequence data shown in Fig. 3 and for providing manuscripts before publication. This work was supported by Public Health Service grant AI 06966 from the National Institute of Allergy and Infections Disease (to A.M.).
Funders:
Funding AgencyGrant Number
National Institute of Allergy and Infections Disease AI 06966
Record Number:CaltechAUTHORS:20120628-074326955
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20120628-074326955
Official Citation:Regulatory region of the heat shock-inducible capR (lon) gene: DNA and protein sequences. R C Gayda, P E Stephens, R Hewick, J M Schoemaker, W J Dreyer, and A Markovitz J. Bacteriol. April 1985 162:271-275
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:32165
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:28 Jun 2012 15:00
Last Modified:28 Jun 2012 15:00

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