Davis, Ronald L. and Davidson, Norman (1984) Isolation of the Drosophila melanogaster dunce chromosomal region and recombinational mapping of dunce sequences with restriction site polymorphisms as genetic markers. Molecular and Cellular Biology, 4 (2). pp. 358-367. ISSN 0270-7306. PMCID PMC368703. http://resolver.caltech.edu/CaltechAUTHORS:20120629-110348691
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Using the method of chromosomal walking, we have isolated a contiguous region of the Drosophila melanogaster X chromosome which corresponds to salivary gland chromosome bands 3C12 to 3D4. This five-band region contains approximately 100 kilobases of DNA, including those sequences comprising dunce, a gene which functions in memory and cyclic nucleotide metabolism. Genome blots of DNA from flies carrying several different chromosomal aberrations with breakpoints in the region have been probed with the isolated clones to map the breakpoints on the cloned DNA and to delimit dunce sequences. This has localized dunce to a 50-kilobase region. In addition, we have searched this 50-kilobase region for restriction site polymorphisms between X chromosomes from different Drosophila strains by genome blotting experiments, and we have followed the segregation of detected polymorphisms and dunce alleles after meiotic recombination. The data map one dunce mutation between two polymorphisms located 10 to 12 kilobases apart.
|Additional Information:||© 1984 American Society for Microbiology. Received 15 August 1983; Accepted 31 October 1983. We are grateful to S. Artavanis-Tsakonas, M. Muskavitch, and D. Hogness for the gift of phage clones. L. Kauvar performed cyclic AMP phosphodiesterase assays, and we are indebted to him. We thank our many colleagues for advice. This research was supported by a grant from the National Institute of Health (NIH) to N.D. Work conducted at Michigan State was supported by NIH Biomedical Research Support Funds and an NIH grant to R.L.D. At Caltech, R.L.D. was supported by postdoctoral fellowships from the Damon Runyon-Walter Winchell Cancer Fund and the NIH.|
|PubMed Central ID:||PMC368703|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Jason Perez|
|Deposited On:||02 Jul 2012 15:49|
|Last Modified:||04 Apr 2017 23:28|
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