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Identification of homo-oligomers as potential intermediates in acetylcholine receptor subunit assembly

Anderson, David J. and Blobel, Günter (1983) Identification of homo-oligomers as potential intermediates in acetylcholine receptor subunit assembly. Proceedings of the National Academy of Sciences of the United States of America, 80 (14). pp. 4359-4363. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:20120710-083723345

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Abstract

We have examined the sedimentation behavior, on sucrose density gradients, of acetylcholine receptor (AcChoR) subunits synthesized in vitro and integrated into heterologous rough microsomal membranes. In media containing nondenaturing detergents such as Triton X-100 or deoxycholate, the subunits appear to self-associate although, as previously reported, no heterologous interactions were detected. The sedimentation profiles assume a broad distribution in the region of 7-13 S. However, the peak fractions occupy the same region of the gradient as does native AcChoR, run in parallel. Such large homo-oligomers were not observed for another membrane protein, opsin, studied in the same way. This indicated that the associations are indeed between the AcChoR subunits and not simply between all newly synthesized membrane proteins. The homologous associations are interpreted to suggest a mechanism for maintaining the ionophore surfaces of the subunits in an energetically preferred, but metastable, configuration during the lengthy period of post-translational assembly.


Item Type:Article
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http://www.pnas.org/content/80/14/4359.abstractPublisherUNSPECIFIED
Additional Information:© 1983 by the National Academy of Sciences. Communicated by Philip Siekevitz, April 4, 1983. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact. We are grateful to Drs. Reid Gilmore, Arthur Karlin, and Ari Helenius for helpful discussions during the course of these experiments. This work was supported by a grant from the Muscular Dystrophy Foundation.
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Funding AgencyGrant Number
Muscular Dystrophy Foundation UNSPECIFIED
Subject Keywords:multi-subunit membrane protein; detergent solubilization; sucrose gradient analysis; homologous associations; early biosynthetic events
Record Number:CaltechAUTHORS:20120710-083723345
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20120710-083723345
Official Citation: D J Anderson and G Blobel Identification of homo-oligomers as potential intermediates in acetylcholine receptor subunit assembly PNAS 1983 80 (14) 4359-4363
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:32317
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:10 Jul 2012 16:33
Last Modified:14 Nov 2014 19:21

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