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Cellular RNA Homologous to the Abelson Murine Leukemia Virus Transforming Gene: Expression and Relationship to the Viral Sequence

Wang, Jean Yin Jen and Baltimore, David (1983) Cellular RNA Homologous to the Abelson Murine Leukemia Virus Transforming Gene: Expression and Relationship to the Viral Sequence. Molecular and Cellular Biology, 3 (5). pp. 773-779. ISSN 0270-7306. http://resolver.caltech.edu/CaltechAUTHORS:20120712-124936634

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Abstract

To examine the expression of the cellular homolog of the Abelson murine leukemia virus transforming gene (the v-abl sequence), a DNA probe representing the v-abl sequence was prepared. The probe detected two cytoplasmic polyadenylic acid-containing c-abl RNAs of about 6.5 and 5.5 kilobases in a variety of rodent cells, and slightly larger RNAs were detected in human cells. These two RNA species were found in all normal tissues or cell lines examined, but at differing concentrations: liver cells had the least, fibroblastic cell lines had the most. By using a probe able to detect the cellular but not the viral gene, the two RNAs were shown to be present in Abelson murine leukemia virus-transformed cells at levels found either in their untransformed counterparts or in similar cell types transformed by other means. The target cells of the virus have a somewhat elevated level of the two RNAs although expression of the c-abl gene is not restricted to these cells. The v-abl sequence lacks 0.35 and 0.85 kilobases of the c-abl RNA on the 5' and 3' ends, respectively. Thus, the Abelson murine leukemia virus transforming gene is an internal fragment of the transcript of a normal cellular gene.


Item Type:Article
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http://mcb.asm.org/content/3/5/773PublisherUNSPECIFIED
Additional Information:© 1983 American Society for Microbiology. Received 15 November 1982; Accepted 27 January 1983. This work was supported by National Cancer Institute grant CA26717 and a National Cancer Institute Core Grant CA14051 (to S. E. Luria). J.Y.J.W. is a postdoctoral fellow of the Jane Coffin Childs Memorial Fund for Medical Research. D.B. is an American Cancer Society Research Professor.
Funders:
Funding AgencyGrant Number
National Cancer InstituteCA26717
National Cancer InstituteCA14051
Record Number:CaltechAUTHORS:20120712-124936634
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20120712-124936634
Official Citation:Cellular RNA homologous to the Abelson murine leukemia virus transforming gene: expression and relationship to the viral sequence. J Y Wang and D Baltimore Mol. Cell. Biol. May 1983 3:773-779; doi:10.1128/MCB.3.5.773
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:32394
Collection:CaltechAUTHORS
Deposited By: Aucoeur Ngo
Deposited On:12 Jul 2012 21:46
Last Modified:12 Jul 2012 21:46

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