Lester, Henry A. and Nerbonne, Jeanne M. (1982) Physiological and Pharmacological Manipulations with Light Flashes. Annual Review of Biophysics and Bioengineering, 11 . pp. 151-175. ISSN 0084-6589 http://resolver.caltech.edu/CaltechAUTHORS:20120717-132820326
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In the experiments described here, a physiological measurement is made while photochemical procedures are employed to alter (a) the concentration of a ligand near membranes or proteins or (b) the structure of the ligand-receptor complexes. Because photochemical reactions often provide the quickest way to produce such chemical perturbations, we emphasize the kinetic information that such experiments have yielded. This information requires a suitably rapid physiological measurement, usually an electrical or optical one. The results often complement those obtained with other kinds of kinetic investigation (iontophoretic application of drugs, stopped-flow mixing, temperature jump, etc). Pharmacological manipulations with light flashes are especially useful for biological systems that cannot be flowed, for instance membranes under electro-physiological investigation or solutions at very low temperatures.
|Additional Information:||© 1982 by Annual Reviews. Research in our laboratory is supported by grants from the Muscular Dystrophy Association, the Pew Foundation, and the National Institutes of Health (NS-11756 and GM-29836 and Career Development Award NS-272 to H.A.L.). J.M.N. has been supported by postdoctoral fellowships from the National Institutes of Health and the American Heart Association. During the preparation of this review, H.A.L. enjoyed the hospitality of A. Levitzki and his colleagues in the Department of Biological Chemistry, Hebrew University of Jerusalem.|
|Official Citation:||Physiological and Pharmacological Manipulations with Light Flashes Annual Review of Biophysics and Bioengineering Vol. 11: 151-175 (Volume publication date June 1982) DOI: 10.1146/annurev.bb.11.060182.001055|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Ruth Sustaita|
|Deposited On:||17 Jul 2012 21:01|
|Last Modified:||17 Jul 2012 21:01|
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