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Studies on cultured Schwann cells: the induction of myelin synthesis, and the control of their proliferation by a new growth factor

Brockes, J. P. and Fryxell, K. J. and Lemke, G. E. (1981) Studies on cultured Schwann cells: the induction of myelin synthesis, and the control of their proliferation by a new growth factor. Journal of Experimental Biology, 95 . pp. 215-230. ISSN 0022-0949. http://resolver.caltech.edu/CaltechAUTHORS:20120717-145448018

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Abstract

We have recently described the use of immunological methods to identify and purify rat Schwann cells. In dissociated cultures of neonatal sciatic nerve, all of the cells can be identified by antigenic criteria as either Schwann cells or fibroblasts. The fibroblasts may be removed by treatment with antiserum to the Thy-1 antigen and complement. The purified Schwann cells have been used to study the regulation of the expression of myelin components, and the stimulation of Schwann cell division by a soluble growth factor. Among the components of myelin, we have concentrated on the peripheral myelin glycoprotein P_0, which constitutes 50–60% of the protein in peripheral myelin. We have studied the distribution of P_0 in vitro and in vivo by immunofluorescence, immuno-autoradiography on SDS gels, and solid-phase radioimmunoassay. Our results support the hypothesis that P_0 is induced specifically as a consequence of the interaction between the Schwann cell and the myelinated type of axon. The level of P_0 in the myelin membrane is at least 1000-fold higher than in the Schwann cell membrane. Purified Schwann cells divide very slowly in a conventional tissue culture medium. This has allowed us to purify a new growth factor from extracts of brain and pituitary, tentatively named Glial Growth Factor (GGF). The activity resides in a basic protein with a native molecular weight of 6 × 10^4 daltons and a subunit molecular weight of 3 × 10^4 daltons, which is active at levels comparable to those of epidermal growth factor. GGF is mitogenic for Schwann cells, astrocytes and muscle fibroblasts.


Item Type:Article
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http://jeb.biologists.org/content/95/1/215.abstractPublisherUNSPECIFIED
Additional Information:© 1981 Company of Biologists. We thank D. R. Balzer Jr. and T. Stevens for their skillful assistance, and Dr K. Stygall for her help in the derivation of monoclonal antibodies to GGF. Research at the California Institute of Technology was supported by grants from the Pew Memorial Trust and the Kroc Foundation, by NIH grant ROI NS 14403 and by Biomedical Research Support grant RR 07003.
Funders:
Funding AgencyGrant Number
Pew Memorial TrustUNSPECIFIED
Kroc FoundationUNSPECIFIED
NIHROI NS 14403
Biomedical Research Support GrantRR 07003
Record Number:CaltechAUTHORS:20120717-145448018
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20120717-145448018
Official Citation: J. P. Brockes, K. J. Fryxell and G. E. Lemke Studies on cultured Schwann cells: the induction of myelin synthesis, and the control of their proliferation by a new growth factor J Exp Biol 1981 95:215-230.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:32526
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:17 Jul 2012 22:20
Last Modified:26 Dec 2012 15:35

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