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Genome structure of Abelson murine leukemia virus variants: proviruses in fibroblasts and lymphoid cells

Goff, Stephen P. and Witte, Owen N. and Gilboa, Eli and Rosenberg, Naomi and Baltimore, David (1981) Genome structure of Abelson murine leukemia virus variants: proviruses in fibroblasts and lymphoid cells. Journal of Virology, 38 (2). pp. 460-468. ISSN 0022-538X. http://resolver.caltech.edu/CaltechAUTHORS:20120718-102042825

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Abstract

We have prepared full-length DNA clones of the Abelson murine leukemia virus (A-MuLV) genome. A specific probe homologous to the central portion of the A-MuLV genome was prepared by nick translation of a subcloned restriction fraction from the cloned DNA. The probe was used to examine the genome structure of several A-MuLV variants. The conclusions are: (i) three viruses coding for Abelson-specific proteins of molecular weight 120,000, 100,000, and 90,000 had genomes indistinguishable in size, suggesting that the shorter proteins are the result of early translational termination; (ii) compared with the genome encoding the 120,000-dalton (120K) protein, a genome coding for a 160K protein was 0.8 kilobase larger in the A-MuLV-specific region; and (iii) a genome coding for a 92K protein had a 700-base pair deletion internal to the coding region. This mutant was transformation defective: its 92K protein lacked the protein kinase activity normally associated with the A-MuLV protein, and cells containing the virus were not morphologically transformed. In addition, we determined the number of A-MuLV proviruses in each of several transformed fibroblast and lymphoid cells prepared by infection in vitro. These experiments show that a single copy of the A-MuLV provirus is sufficient to transform both types of cells and that nonproducer cells generally have only one integrated provirus.


Item Type:Article
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URLURL TypeDescription
http://jvi.asm.org/content/38/2/460.abstractPublisherUNSPECIFIED
Additional Information:© 1981 American Society for Microbiology. Received 25 November 1980a; Accepted 30 January 1981. This work was supported by Public Health Service grant CA-24220 (to N.R.), program project grant CA-24530 (to N.R.), grant CA-26717 (to D.B.), and grant CA-14051 (core grant to S. E. Luria), all from the National Cancer Institute. S.P.G. was a postdoctoral fellow of the Jane Coffin Childs Memorial Fund for Medical Research. O.N.W. was a postdoctoral fellow of the Helen Hay Whitney Foundation. N.R. is a recipient of a Research Scholar Award from the American Cancer Society (Massachusetts division). D.B. is an American Cancer Society research Professor.
Funders:
Funding AgencyGrant Number
National Cancer Institute Public Health Service grantCA-24220
National Cancer InstituteCA-24530
National Cancer InstituteCA-26717
National Cancer InstituteCA-14051
Jane Coffin Childs Memorial Fund for Medical Research postdoctoral fellowshipUNSPECIFIED
Helen Hay Whitney Foundation postdoctoral fellowshipUNSPECIFIED
American Cancer Society research scholar awardUNSPECIFIED
Record Number:CaltechAUTHORS:20120718-102042825
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20120718-102042825
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:32547
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:18 Jul 2012 17:53
Last Modified:18 Jul 2012 17:53

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