Shoemaker, Charles and Goff, Stephen and Gilboa, Eli and Paskind, Michael and Mitra, Sudha W. and Baltimore, David (1980) Structure of a cloned circular Moloney murine leukemia virus DNA molecule containing an inverted segment: implications for retrovirus integration. Proceedings of the National Academy of Sciences of the United States of America, 77 (7). pp. 3932-3936. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:20120719-130834887
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Closed circular Moloney murine leukemia virus (M-MuLV) DNA was prepared from recently infected cells and cloned in a lambda vector. Four classes of cloned M-MuLV inserts were found: Class I, full length 8.8-kilobase (kb) inserts with two tandem long terminal repeats (LTRs) of 600 base pairs; class 2, 8.2-kb inserts with a single copy of a LTR; class 3, M-MuLV DNA inserts with various portions deleted; and class 4, an 8.8-kb insert with an internal sequence inversion. Determination of nucleotide sequence at the junction between the two LTRs from a class 1 insert suggested that circularization occurred by blunt-end ligation of an 8.8-kb linear DNA. The class 4 molecule had an inversion that was flanked by inverted LTRs, each of which had lost two terminal base pairs at the inversion end points. Also, four base pairs that were present only once in standard M-MuLV DNA were duplicated at either end of the inversion. This molecule was interpreted as resulting from an integrative inversion in which M-MuLV DNA has integrated into itself. Its analysis thus provided explicit information concerning the mechanism by which retrovirus DNA integrates into host cell DNA. Models of retrovirus integration based on bacterial DNA transposition mechanisms are proposed.
|Additional Information:||© 1980 National Academy of Sciences. Contributed by David Baltimore, April 14, 1980. This work was supported by Grants CA-14051 and CA-26717 from the National Cancer Institute and Contract N01-CP-53562 from the Division of Cancer Cause and Prevention, National Cancer Institute. S.G. is a Postdoctoral Fellow of the Jane Coffin Childs Memorial Fund for Medical Research. S.W.M. is a Postdoctoral Fellow of the American Cancer Society, Massachusetts Division. D.B. is an American Cancer Society Research Professor. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U. S. C. §1734 solely to indicate this fact.|
|Subject Keywords:||transposable DNA elements; long terminal repeats|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Jason Perez|
|Deposited On:||19 Jul 2012 21:50|
|Last Modified:||25 Mar 2014 17:22|
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