Witte, Owen N. and Ponticelli, Alfred and Gifford, Ann and Baltimore, David and Rosenberg, Naomi and Elder, John (1981) Phosphorylation of the Abelson Murine Leukemia Virus Transforming Protein. Journal of Virology, 39 (3). pp. 870-878. ISSN 0022-538X http://resolver.caltech.edu/CaltechAUTHORS:20120719-161708980
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The Abelson murine leukemia virus transforming gene product is a phosphorylated protein encoded by both viral and cellular sequences. This gene product has an amino-terminal region derived from the gag gene of its parent virus and a carboxyl-terminal region (abl) derived from a normal murine cellular gene. Using a combination of partial proteolytic cleavage techniques and antisera specific for gag and abl sequences, we mapped in vivo phosphorylation sites to different regions of the protein. Phosphoproteins encoded by strain variants and transformation-defective mutants of Abelson murine leukemia virus with defined deletions in the primary sequence of the abl region were compared by two-dimensional limit digest peptide mapping. Specific phosphorylation pattern differences for wild-type and mutant proteins probably represented deletions of specific phosphate acceptor sites in the abl region. An in vitro autophosphorylation activity copurified with the Abelson murine leukemia virus protein from transformation-competent strains. A peptide analysis of such in vitro reactions demonstrated that these phosphorylation sites were restricted to the aminoterminal region, and the specific sites appeared to be unrelated to the sites found on proteins phosphorylated in vivo. Thus, the autophosphorylation reaction probably correlates with an activity important in transformation, but the specific end product in vitro bears little resemblance to its function in vivo.
|Additional Information:||© 1981 American Society for Microbiology. Received 23 March 1981; Accepted 13 May 1981. This work was supported by Public Health Service grants CA27507 (to O.N.W.), CA24220 (to N.R.), CA24530 (to R. Schwartz), and CA14051 (to S. Luria) from the National Institutes of Health, by grants MV-34L (to D.B.) and IN-131 (to R. Steckel) from the American Cancer Society, and by a grant from the California Institute for Cancer Research (to O.N.W.). N.R. is a recipient of an American Cancer Society Massachusetts Division Research Scholar Award. D.B. is an American Cancer Society Research Professor. We gratefully acknowledge the photographic assistance of Michael Paskind and Joy Stafford.|
|Official Citation:||Phosphorylation of the Abelson murine leukemia virus transforming protein. O N Witte, A Ponticelli, A Gifford, D Baltimore, N Rosenberg, and J Elder J. Virol. September 1981 39:870-878|
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|Deposited By:||Aucoeur Ngo|
|Deposited On:||20 Jul 2012 15:15|
|Last Modified:||20 Jul 2012 15:15|
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