Baltimore, David and Smoler, Donna (1971) Primer Requirement and Template Specificity of the DNA Polymerase of RNA Tumor Viruses. Proceedings of the National Academy of Sciences of the United States of America, 68 (7). pp. 1507-1511. ISSN 0027-8424 http://resolver.caltech.edu/CaltechAUTHORS:20120724-132033639
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Polyribonucleotides will act as efficient templates for the DNA polymerases found in the virions of avian myeloblastosis virus and mouse leukemia virus if a short complementary oligodeoxyribonucleotide primer is added. Synthesis of the complementary polydeoxyribonucleotide continues until an amount of polymer equal to the amount of initial template has been produced. The two viruses show slightly different specificities toward the four homoribopolymers. Polydeoxyribonucleotides are generally much poorer templates than the homologous polyribonucleotides, in most cases yielding no detectable synthesis. The DNA polymerase of RNA tumor viruses, therefore, have the same requirements for activity as do other DNA polymerases, except that they prefer polyribonucleotides over polydeoxyribonucleotides as templates.
|Additional Information:||© 1971 National Academy of Sciences. Communicated by Alexander Rich, April 26, 1971. We thank Drs. F. Bollum, O. Friedman, J. Huberman, and A. Kornberg for providing materials used in this study and for advice about their properties. We thank Dr. I. Verma for his help during this work. We have been supported by grants no. AI-08388 from the USPHS and grant no. E-512 from the American Cancer Society. D. B. is a Faculty Research Awardee of the American Cancer Society.|
|Subject Keywords:||avian myeloblastosis virus; mouse leukemia virus; E. coli DNA polymerase; homopolynucleotides; oligonucleotides|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Jason Perez|
|Deposited On:||24 Jul 2012 23:14|
|Last Modified:||24 Jul 2012 23:14|
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