Datta, B. and Poddar, R. K. (1970) Greater Vulnerability of the Infecting Viral Strand of Replicative-Form Deoxyribonucleic Acid of Bacteriophage φX174. Journal of Virology, 6 (5). pp. 583-588. ISSN 0022-538X. http://resolver.caltech.edu/CaltechAUTHORS:20120725-114743447
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Four types of φX-infected cells of Escherichia coli CR, a thymine-requiring strain of E. coli C, were prepared in which the parental replicative-form deoxyribonucleic acid (RF DNA) was labeled with same specific amounts of bromouracil in (i) both strands, (ii) only the infecting viral strand, (iii) only the complementary strand, and (iv) neither strand. The sensitivity of each type of infected cell toward irradiation by ultraviolet light, visible light, and X rays was measured. The results indicate that a certain amount of radiation damage in the infecting viral strand of the parental RF was more inhibitory to the production of progeny phage than when the damage was in the complementary strand. Similar conclusions were also drawn from “suicide” experiments of the phage-infected complexes containing ^(32)P of the same specific activity on either strand of the parental RF DNA. The results suggest that the beta decay occurring in the infecting viral strand was more effective in inactivating the plaque-forming ability of the complex.
|Additional Information:||© 1970 American Society for Microbiology. Received for publication 25 June 1970. This work was carried out with the financial assistance of the Department of Atomic Energy and the Council of Scientific and Industrial Research, Government of India. It is a pleasure to acknowledge the sincere cooperation of S. R. Palchaudhury and U. Chaudhuri during the course of this work. We are grateful to R. L. Sinsheimer and F. W. Stahl for going through the manuscript and for their helpful criticism.|
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|Deposited By:||Jason Perez|
|Deposited On:||25 Jul 2012 20:17|
|Last Modified:||23 Aug 2016 10:14|
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