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Location of the 5-Methylcytosine Group on the Bacteriophage φX174 Genome

Lee, Amy Shiu and Sinsheimer, Robert L. (1974) Location of the 5-Methylcytosine Group on the Bacteriophage φX174 Genome. Journal of Virology, 14 (4). pp. 872-877. ISSN 0022-538X. http://resolver.caltech.edu/CaltechAUTHORS:20120726-100159402

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Abstract

Bacteriophage φX174 DNA was labeled in vivo with [methyl-^(3)H]methionine. The methyl-labeled progeny DNA was extracted from purified bacteriophage φX174 particles and was used as template for in vitro synthesis of the complementary strand in the presence of the nucleoside triphosphates and Escherichia coli polymerase I. The resultant replicative form DNA was then cleaved, in separate experiments, with restriction endonucleases from Haemophilus influenzae and H. aegyptius. The DNA fragments were analyzed by polyacrylamide gel electrophoresis. It is concluded that the single methylcytosine in the viral DNA is located in a specific region of the φX174 genome, very likely in gene H.


Item Type:Article
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http://jvi.asm.org/content/14/4/872.abstractPublisherUNSPECIFIED
Additional Information:© 1974 American Society for Microbiology. Received for publication 6 June 1974. This work was supported by Public Health Service Grant GM13554 from the National Institute of General Medical Sciences.
Funders:
Funding AgencyGrant Number
National Institute of General Medical Sciences Public Health Service (PHS)GM13554
Record Number:CaltechAUTHORS:20120726-100159402
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20120726-100159402
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:32744
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:30 Jul 2012 16:09
Last Modified:30 Jul 2012 16:09

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