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Process of Infection with Bacteriophage φX174. XXXVI. Measurement of Virus-Specific Proteins During a Normal Cycle of Infection

Mayol, Robert F. and Sinsheimer, Robert L. (1970) Process of Infection with Bacteriophage φX174. XXXVI. Measurement of Virus-Specific Proteins During a Normal Cycle of Infection. Journal of Virology, 6 (3). pp. 310-319. ISSN 0022-538X. http://resolver.caltech.edu/CaltechAUTHORS:20120726-131302985

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Abstract

Double-labeling techniques in which ^(14)C-labeled, φX174-infected cells and ^(3)H-labeled, uninfected cells were used permitted the identification of the virus-specific proteins after separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis without prior inhibition of host-cell protein synthesis by ultraviolet irradiation. It was also possible to detect previously undescribed components of high molecular weight which may represent induced host proteins. The gel regions specifically corresponding to cistron II protein and the chloramphenicol-resistant VI protein were identified, and a third new, small peak of unknown origin was detected. Studies of the rate of synthesis of virus-specific proteins at various times after infection indicated that the product of cistron I (lysis) is made only late in infection, but the other proteins seemed to be synthesized at the same relative rates throughout infection (although in different amounts). Studies of the proteins obtained from uniformly labeled φX virus particles indicated that all of the spikes are identical and allowed a formulation of the structure of the phage capsid.


Item Type:Article
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http://jvi.asm.org/content/6/3/310.abstractPublisherUNSPECIFIED
Additional Information:© 1970 American Society for Microbiology. Received for publication 24 April 1970. This work was supported by Public Health Service grant GM 13554 from the National Institute of General Medical Sciences.
Funders:
Funding AgencyGrant Number
National Institute of General Medical Sciences Public Health Service grantGM 13554
Record Number:CaltechAUTHORS:20120726-131302985
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20120726-131302985
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ID Code:32751
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:30 Jul 2012 15:58
Last Modified:30 Jul 2012 15:58

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