CaltechAUTHORS
  A Caltech Library Service

Covalent Linkage Between Ribonucleic Acid Primer and Deoxyribonucleic Acid Product of the Avian Myeloblastosis Virus Deoxyribonucleic Acid Polymerase

Verma, Inder M. and Meuth, Nora L. and Baltimore, David (1972) Covalent Linkage Between Ribonucleic Acid Primer and Deoxyribonucleic Acid Product of the Avian Myeloblastosis Virus Deoxyribonucleic Acid Polymerase. Journal of Virology, 10 (4). pp. 622-627. ISSN 0022-538X. http://resolver.caltech.edu/CaltechAUTHORS:VERjvir72

[img]
Preview
PDF
See Usage Policy.

975Kb

Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:VERjvir72

Abstract

Initiation of deoxyribonucleic acid (DNA) synthesis by the avian myeloblastosis virus DNA polymerase was previously suggested to involve a ribonucleic acid (RNA) primer, the initial product being a DNA molecule joined by a phosphodiester bond to the RNA primer. The existence and nature of such an RNA-DNA joint was investigated by assaying for transfer of a 32P atom from an {alpha}-32P-deoxyribonucleotide to a 2'(3')-ribonucleotide after alkaline hydrolysis of the polymerase product. Such a transfer was observed, but only from {alpha}-32P-deoxyadenosine triphosphate and only to 2'(3')-adenosine monophosphate. This same transfer was observed in both the endogenous DNA polymerase reaction of purified virions and the reconstructed reaction of purified DNA polymerase plus purified 60 to 70S viral RNA. These results indicate a high level of specificity for the initiation process and support the idea of a low-molecular-weight initiator RNA as part of the 60 to 70S RNA complex.


Item Type:Article
Additional Information:Copyright © 1972 American Society for Microbiology. Received for publication 13 June 1972 We are grateful to Ian Molineux for helpful suggestions. This work was supported by a grant from the American Cancer Society and a contract from Special Virus Cancer Program of the National Cancer Institute. I. M. Verma was a fellow of the Jane Coffin Childs Memorial Fund for Medical Research and D. Baltimore was a faculty research awardee of the American Cancer Society.
Record Number:CaltechAUTHORS:VERjvir72
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:VERjvir72
Alternative URL:http://jvi.asm.org/cgi/content/abstract/10/4/622
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:3284
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:25 May 2006
Last Modified:26 Dec 2012 08:53

Repository Staff Only: item control page