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Quantitation of avian RNA tumor virus reverse transcriptase by radioimmunoassay

Panet, Amos and Baltimore, David and Hanafusa, Teruko (1975) Quantitation of avian RNA tumor virus reverse transcriptase by radioimmunoassay. Journal of Virology, 16 (1). pp. 146-152. ISSN 0022-538X.

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A radioimmunoassay was developed that can detect and quantitate 3 ng or more of the avian RNA tumor virus reverse transcriptase. The assay detected no antigenic sites in Rous sarcoma virus alpha virions or in virions of a murine RNA tumor virus. About 70 molecules of reverse transcriptase were found per virion of avian myleloblastosis virus with this assay or with an assay based on antibody inhibition of enzymatic activity. The assay detected about 270 ng of enzyme per mg of cell protein in virus-producing cells; uninfected cells had much less antigenic material but contained some determinants able to displace radioactive antigen. No additional antigenic determinants on reverse transcriptase could be detected that were not found on the separated alpha subunit of the enzyme. Although sevenfold less sensitive than enzymatic activity as a measure of reverse transcriptase, the radioimmunoassay can detect antigen using small amounts of protein and in the presence of inhibtors.

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Additional Information:© 1975 American Society for Microbiology. Received for publication 19 February 1975. This investigation was supported by a Public Health Service contract from the Virus Cancer Program of the National Cancer Institute and Public Health Service grants CA 14051 and CA 14936 from the National Cancer Institute. D. B. was supported by a Research Professorship from the American Cancer Society. We wish to thank R. Nowinski and J. W. Beard for materials.
Funding AgencyGrant Number
National Cancer Institute Virus Cancer ProgramUNSPECIFIED
National Cancer Institute Public Health ServiceCA 14051
National Cancer Institute Public Health ServiceCA 14936
American Cancer SocietyUNSPECIFIED
Record Number:CaltechAUTHORS:20120801-131306461
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ID Code:32842
Deposited By: Jason Perez
Deposited On:01 Aug 2012 22:13
Last Modified:23 Aug 2016 10:15

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