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Identification of a Protein Linked to Nascent Poliovirus RNA and to the Polyuridylic Acid of Negative-Strand RNA

Pettersson, Ralf F. and Ambros, Victor and Baltimore, David (1978) Identification of a Protein Linked to Nascent Poliovirus RNA and to the Polyuridylic Acid of Negative-Strand RNA. Journal of Virology, 27 (2). pp. 357-365. ISSN 0022-538X. http://resolver.caltech.edu/CaltechAUTHORS:20120802-101224844

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Abstract

A protein similar to that previously demonstrated on poliovirus RNA and replicative intermediate RNA (VPg) was found on all sizes of nascent viral RNA molecules and on the polyuridylic acid isolated from negative-strand RNA. ^(32)P-labeled nascent chains were released from their template RNA and fractionated by exclusion chromatography on agarose. Fingerprint analysis using two-dimensional polyacrylamide gels of RNase T1 oligonucleotides derived from nascent chains of different lengths showed that a size fractionation of nascent chains was achieved. VPg was recovered from nascent chains varying in length from 7,500 nucleotides (full-sized RNA) to about 500 nucleotides. No other type of 5' terminus could be demonstrated on nascent RNA, and the yield of VPg was consistent with one molecule of the protein on each nascent chain. These results are consistent with the concept that the protein is added to the 5' end of the growing RNA chains at a very early stage, possibly as a primer of RNA synthesis. Analysis of the polyuridylic acid tract isolated from the replicative intermediate and double-stranded RNAs indicated that a protein of the same size as that found on the nascent chains and virion RNA is also linked to the negative-strand RNAs. It is likely that a similar mechanism is responsible for initiation of synthesis of both plus- and minus-strand RNAs.


Item Type:Article
Related URLs:
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http://jvi.asm.org/content/27/2/357.abstractPublisherUNSPECIFIED
Additional Information:© 1978 American Society for Microbiology. Received for publication 26 January 1978. This work was supported by Public Health Service grant AI-08388 from the National Institute of Allergy and Infectious Diseases and grants CA-14051 and CA-12174 from the National Cancer Institute. R.F.P. was a recipient of Public Health Service international fellowship F05 TW-2245 from the Fogarty International Center. D.B. is an American Cancer Society Research Professor.
Funders:
Funding AgencyGrant Number
National Institute of Allergy and Infectious Diseases Public Health ServiceAl-08388
National Cancer Institute Public Health Service CA-14051
National Cancer Institute Public Health Service CA-12174
Fogarty International Center Public Health Service International FellowshipF05 TW-2245
Record Number:CaltechAUTHORS:20120802-101224844
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20120802-101224844
Official Citation:Identification of a protein linked to nascent poliovirus RNA and to the polyuridylic acid of negative-strand RNA. R F Pettersson, V Ambros, and D Baltimore J. Virol. August 1978 27:357-365
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:32875
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:02 Aug 2012 18:12
Last Modified:02 Aug 2012 18:12

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