Silverman, Michael and Simon, Melvin (1974) Positioning Flagellar Genes in Escherichia coli by Deletion Analysis. Journal of Bacteriology, 117 (1). pp. 73-79. ISSN 0021-9193. PMCID PMC246526. http://resolver.caltech.edu/CaltechAUTHORS:20120803-095603378
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Two methods were devised to select a series of overlapping deletion mutations carried on episomal elements in Escherichia coli. The deletions were then used in an analysis of (i) the relative position on the genome of previously described mutant loci in the flagellar genes, (ii) the relative position of a newly defined cistron, flaN, and (iii) the orientation and direction of transcription of genes previously assigned to multicistronic transcriptional units. As a result of this analysis and previous work, we report the following arrangement of the flagellar genes in the his-aroD region of the E. coli genetic map: his-flaR-flaQ-flaP-flaA-flaE- flaO-flaC-flaB-flaN-hag-flaD-uvrC-flaI-mot-flaG-flaH. The genes flaA, P, Q, and R, flaB, C, 0 and E, and flaG and H are co-transcribed in that order.
|Additional Information:||© 1974 American Society for Microbiology. Received for publication 27 September 1973. The cooperation of Julius Adler in providing the nonchemotactic strain is appreciated. M. Silverman was supported by funds from the Cancer Coordinating Committee of the University of California, San Diego. This investigation was supported by a research grant, GB-15655, from the National Science Foundation.|
|PubMed Central ID:||PMC246526|
|Official Citation:||Positioning Flagellar Genes in Escherichia coli by Deletion Analysis Michael Silverman and Melvin Simon J. Bacteriol. January 1974 117:73-79|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Aucoeur Ngo|
|Deposited On:||03 Aug 2012 17:31|
|Last Modified:||05 Apr 2017 16:44|
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