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Replication of Sindbis Virus V. Polyribosomes and mRNA in Infected Cells

Simmons, Daniel T. and Strauss, James H. (1974) Replication of Sindbis Virus V. Polyribosomes and mRNA in Infected Cells. Journal of Virology, 14 (3). pp. 552-559. ISSN 0022-538X. http://resolver.caltech.edu/CaltechAUTHORS:20120807-160935284

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Abstract

Cells infected with wild-type Sindbis virus contain at least two forms of mRNA, 26S and 49S RNA. Sindbis 26S RNA (molecular weight 1.6 x 10^6) constitutes 90% by weight of the mRNA in infected cells, and is thought to specify the structural proteins of the virus. Sindbis 49S RNA, the viral genome (molecular weight 4.3 x 10^6), constitutes approximately 10% of the mRNA in infected cells and is thought to supply the remaining viral functions. In cells infected with ts2, a temperature-sensitive mutant of Sindbis virus, the messenger forms also include a third species of RNA with a sedimentation coefficient of 33S and an apparent molecular weight of 2.3 x 10^6. Hybridization-competition experiments showed that 90% of the base sequences in 33S RNA from these cells are also present in 26S RNA. Sindbis 33S RNA was also isolated from cells infected with wild-type virus. After reaction with formaldehyde, this species of 33S RNA appeared to be completely converted to 26S RNA. These results indicate that 33S RNA isolated from cells infected with either wild-type Sindbis or ts2 is not a unique and separate form of Sindbis RNA.


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Additional Information:© 1974 American Society for Microbiology. Received for publication 17 April 1974. We wish to acknowledge the expert technical assistance of Edith M. Lenches and Sharman E. S. Christoph in preparing the cultures of chicken embryo fibroblasts. We also wish to thank Ellen G. Strauss for helpful suggestions in writing this manuscript. This investigation was supported in part by the National Science Foundation grant no. GB 31763X and by the Public Health Service grant no. GM 06965 from the National Institute of General Medical Sciences. D.T.S. was supported by Public Health Service Training grant no. 5-T1-GM-86 from the National Institute of General Medical Sciences.
Funders:
Funding AgencyGrant Number
NSFGB 31763X
Public Health Service (PHS)GM 06965
Public Health Service Training Grant5-T1-GM-86
Record Number:CaltechAUTHORS:20120807-160935284
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20120807-160935284
Official Citation:Replication of Sindbis Virus V. Polyribosomes and mRNA in Infected Cells Daniel T. Simmons and James H. Strauss J. Virol. September 1974 14:552-559
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:32995
Collection:CaltechAUTHORS
Deposited By: Aucoeur Ngo
Deposited On:07 Aug 2012 23:27
Last Modified:26 Dec 2012 15:52

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