Storrie, Brian and Attardi, Giuseppe (1973) Expression of the Mitochondrial Genome in HeLa Cells. XV. Effect of Inhibition of Mitochondrial Protein Synthesis on Mitochondrial Formation. Journal of Cell Biology, 56 (3). pp. 819-831. ISSN 0021-9525 http://resolver.caltech.edu/CaltechAUTHORS:20120807-165200102
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The effect of selective inhibition of mitochondrial protein synthesis by chloramphenicol at 40 or 200 µg/ml on the formation of mitochondria in HeLa cells was investigated. HeLa cells, under the conditions used in the present work, grow at a decreasing rate for at least four cell generations in the presence of 40 µg/ml chloramphenicol, and for two generations in the presence of 200 µg/ml chloramphenicol. The progressive cell growth inhibition which begins after 2 days of exposure of the cells to 40 µg/ml chloramphenicol is immediately or gradually reversible, upon removal of the drug, for periods up to at least 8 days of treatment, though there is a progressive loss of cloning efficiency. In cells which have been treated for 6–7 days with 40 or 200 µg/ml of chloramphenicol, mitochondrial protein synthesis occurs at a normal or near-normal rate 1 h after removal of the drug. Mitochondria increase normally in number and show a normal size and amount of cristae in the presence of either concentration of drug. However, in 4–5% of the mitochondrial profiles the cristae appear to be arranged in unusual, circular, looped or whorled configuration.
|Additional Information:||© 1973 Rockefeller University Press. Submitted: 3 August 1972; Revision received 30 October 1972. The valued assistance of Mr. P. Koen with the electron microscopy, of Mr. J. Posakony and Dr. J. England with the DAB reaction, and of Mrs. B. Keeley is gratefully acknowledged. This work was supported by a grant from the U. S. Public Health Service (GM-11726) and by a National Science Foundation predoctoral fellowship to Dr. Storrie. Paper XIV in this series is M. Wu, N. Davidson, G. Attardi, and Y. Aloni, 1972, J. Mol. Biol. 71:81.|
|Official Citation:||Expression of the Mitochondrial Genome in HeLa Cells: XV. Effect of Inhibition of Mitochondrial Protein Synthesis on Mitochondrial Formation Brian Storrie and Giuseppe Attardi J Cell Biol 1973 56:819-831. Published March 1, 1973, doi:10.1083/jcb.56.3.819|
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|Deposited On:||08 Aug 2012 14:13|
|Last Modified:||26 Dec 2012 15:52|
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