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Actin, α-actinin, and tropomyosin interaction in the structural organization of actin filaments in nonmuscle cells

Lazarides, Elias (1976) Actin, α-actinin, and tropomyosin interaction in the structural organization of actin filaments in nonmuscle cells. Journal of Cell Biology, 68 (2). pp. 202-219. ISSN 0021-9525. http://resolver.caltech.edu/CaltechAUTHORS:20120808-091429959

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Abstract

During the spreading of a population of rat embryo cells, approximately 40% of the cells develop a strikingly regular network which precedes the formation of the straight actin filament bundles seen in the fully spread out cells. Immunofluorescence studies with antibodies specific for the skeletal muscle structural proteins actin, α-actinin, and tropomyosin indicate that this network is composed of foci containing actin and α-actinin, connected by tropomyosin-associated actin filaments. Actin filaments, having both tropomyosin and α-actinin associated with them, are also seen to extend from the vertices of this network to the edges of the cell. These results demonstrate a specific interaction of α-actinin and tropomyosin with actin filaments during the assembly and organization of the actin filament bundles of tissue culture cells. The three-dimensional network they form may be regarded as the structural precursor and the vertices of this network as the organization centers of the ultimately formed actin filament bundles of the fully spread out cells.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1083/jcb.68.2.202DOIUNSPECIFIED
http://jcb.rupress.org/content/68/2/202.abstractPublisherUNSPECIFIED
Additional Information:© 1976 Rockefeller University Press. Received for publication 18 June 1975, and in revised form 5 September 1975. I am grateful to Dr. J. D. Watson for his advice and support throughout this work, to Dr. R. Pollack for the generous use of his laboratory facilities, to Dr. G. Albrecht-Buehler for his invaluable advice, and to Dr. F. Miller at the State University of New York at Stony Brook for his advice and help in the preparation of the antibodies. I am indebted to Dr. K. R. Porter at the University of Colorado, Boulder, who showed me that he had observed similar networks in cultured epithelial cells by light microscopy just around the time I was being born. This investigation was supported by the National Institutes of Health, Research Grant CA-13106 from the National Cancer Institute.
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Funding AgencyGrant Number
NIH Research GrantCA-13106
Record Number:CaltechAUTHORS:20120808-091429959
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20120808-091429959
Official Citation:Actin, α-actinin, and tropomyosin interaction in the structural organization of actin filaments in nonmuscle cells. E Lazarides 68:202-219. doi:10.1083/jcb.68.2.202
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:33007
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:08 Aug 2012 16:47
Last Modified:26 Dec 2012 15:53

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