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Direction of Polymerization by the Avian Myeloblastosis Virus Deoxyribonucleic Acid Polymerase

Smoler, Donna and Molineux, Ian and Baltimore, David (1971) Direction of Polymerization by the Avian Myeloblastosis Virus Deoxyribonucleic Acid Polymerase. Journal of Biological Chemistry, 246 (24). pp. 7697-7700. ISSN 0021-9258. http://resolver.caltech.edu/CaltechAUTHORS:20120814-122451361

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Abstract

An oligonucleotide primer is necessary to initiate polymerization of nucleotides by the DNA polymerase of avian myeloblastosis virus. The role of the primer was investigated by synthesizing the acid-soluble oligonucleotide [5'-^(32)P](dT)_(10). Conversion of this primer to an acid-insoluble form by the DNA polymerase in the presence of poly(A) indicated that the primer was physically incorporated into the product. The phosphate residue at the 5'-terminus of the primer remained sensitive to alkaline phosphatase, and synthesis therefore appeared to proceed by addition of mononucleotides at the 3'-OH of the primer. Inhibition of DNA polymerase activity by dideoxythymidine triphosphate was observed using templates containing AMP or when the enzyme was copying the viral RNA found in the virion. Copying of poly(C) by the DNA polymerase was not sensitive to the analogue. These results corroborate the conclusion that the polymerase adds nucleotides to the 3'-OH of a primer and further indicate that copying of the endogenous viral RNA involves only polymerization on 3'-OH groups.


Item Type:Article
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http://www.jbc.org/content/246/24/7697.abstractPublisherUNSPECIFIED
Additional Information:© 1971 American Society of Biological Chemists, Inc. Received August 19, 1971. This work was supported by American Cancer Society Grant VC-4C and a contract from the Special Virus Cancer Program of the National Cancer Institute. Faculty Research Awardee of the American Cancer Society. We are grateful to Drs. Gobind Khorana, Arthur Kornberg, and Inder Verma for generous gifts of materials and advice. We also thank Dr. Malcolm Gefter who assisted in the urification of [5'-^(32)P](dT)_10, and Dr. Joel Huberman for suggesting the use of ddTTP.
Funders:
Funding AgencyGrant Number
American Cancer SocietyVC-4C
National Cancer InstituteUNSPECIFIED
Subject Keywords:Enzymology
Record Number:CaltechAUTHORS:20120814-122451361
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20120814-122451361
Official Citation:Donna Smoler, Ian Molineux, and David Baltimore Direction of Polymerization by the Avian Myeloblastosis Virus Deoxyribonucleic Acid Polymerase J. Biol. Chem. 1971 246: 7697-7700.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:33172
Collection:CaltechAUTHORS
Deposited By: Aucoeur Ngo
Deposited On:14 Aug 2012 19:39
Last Modified:14 Aug 2012 19:39

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