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Morphological heterogeneity of HeLa cell mitochondria visualized by a modified diaminobenzidine staining technique

Posakony, J. W. and England, J. M. and Attardi, G. (1975) Morphological heterogeneity of HeLa cell mitochondria visualized by a modified diaminobenzidine staining technique. Journal of Cell Science, 19 (2). pp. 315-329. ISSN 0021-9533. http://resolver.caltech.edu/CaltechAUTHORS:20120814-142210986

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Abstract

The diaminobenzidine (DAB) technique for the ultrastructural localization of sites of cytochrome c oxidase activity in animal tissues has been adapted to the visualization of mitochondria in animal cells growing in culture. The modified technique allows the staining of mitochondria in all cells in coverslip preparatins for light microscopy. Electron microscopy of thin sections of material treated by this method has revealed that all mitochondrial profiles within a cell (and only these) are stained and they exhibit a well preserved size and internal structure. Coverslip cultures of synchronized and unsynchronized HeLa (F-315) cells stained with the DAB reaction were examined under oil immersion. In the majority of the cells, mitochondria were recognized as discrete bodies in the thinner peripheral portion of the cytoplasm. This observation indicates that in a large proportion of HeLa F-315 cells, at least under the growth conditions used here, the mitochondrial complement is dividied into distinct organelles. This examination also revealed a considerable morphological heterogeneity of mitochondria, which exhibited an ovoid or short rod-like or, less frequently, long filamentous shape, with some evidence of branching. The variability in mitochondrial morphology appeared to be far more prounced between different cells than within individual cells; this cellular heterogeneity was not related in any obvious way to cell-cycle-dependent changes.


Item Type:Article
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http://jcs.biologists.org/content/19/2/315.abstractPublisherUNSPECIFIED
Additional Information:© 1975 by Company of Biologists. Received 28 April 1975. The useful suggestions of Dr M. J. Karnovsky are gratefully acknowledged, as is the excellent technical assistance of Ms B. Keeley. This work was supported by NIH grant GM-11726, a Dernham Fellowship (D 196) of the American Cancer Society (J .E.), and an NSF Summer Research Traineeship (J.P.).
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Funding AgencyGrant Number
NIHGM-11726
American Cancer Society Dernham FellowshipD 196
NSF Summer Research TraineeshipUNSPECIFIED
Record Number:CaltechAUTHORS:20120814-142210986
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20120814-142210986
Official Citation: J.W. Posakony, J.M. England, and G. Attardi Morphological heterogeneity of HeLa cell mitochondria visualized by a modified diaminobenzidine staining technique J Cell Sci 1975 19:315-329
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:33184
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:14 Aug 2012 21:37
Last Modified:26 Dec 2012 15:59

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