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Cell Transformation by Different Forms of Polyoma Virus DNA

Crawford, Lionel and Dulbecco, Renato and Fried, Mike and Montagnier, Luc and Stoker, Michael (1964) Cell Transformation by Different Forms of Polyoma Virus DNA. Proceedings of the National Academy of Sciences of the United States of America, 52 (1). pp. 148-152. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:20120815-100043902

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Abstract

DNA extracted from purified polyoma virus contains closed and open molecules which are separable by velocity sedimentation either in the analytical or in the preparative ultracentrifuge. The closed configuration of the DNA gives rise to the fast band (component I) and the open configuration to a slow band, which is in some cases double (components II and III). Components I and II are able to produce plaques on mouse embryo monolayers, i.e., they possess cytocidal activity but which of the components cause cell transformation could not be determined owing to the lack of an adequate quantitative assay for transformation. A determination of the role of these DNA components in transformation is important, since it may clarify the mechanism of transformation. Such a determination is now made possible by the development of a sensitive quantitative assay for transformation caused by polyoma virus. This method is based on the ability of the transformed cells to form colonies in agar where the vast majority of the untransformed cells are unable to form colonies. Preliminary experiments have demonstrated that this method is also suitable for the study of the transforming ability of polyoma DNA.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1073/pnas.52.1.148DOIUNSPECIFIED
http://www.pnas.org/content/52/1/148PublisherUNSPECIFIED
Additional Information:© 1964 National Academy of Sciences. Communicated May 11, 1964. We are indebted to Miss Anne Smith and Miss Margaret Ballantyne for their excellent technical assistance. One of us (M. F.) was supported in part by grants from the Arthur McCallum Fund and the Nutrition Foundation. This research was conducted at the Institute of Virology, University of Glasgow, by Lionel Crawford and Michael Stoker (Medical Research Council Experimental Virus Research Unit), Renato Dulbecco (Visiting Professor of the Royal Society from Salk Institute for Biological Sciences, San Diego, California), Mike Fried (California Institute of Technology, Pasadena, California), and Luc Montagnier (Laboratoire Pasteur de lInstitut du Radium, Paris).
Funders:
Funding AgencyGrant Number
Arthur McCallum FundUNSPECIFIED
Nutrition FoundationUNSPECIFIED
Record Number:CaltechAUTHORS:20120815-100043902
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20120815-100043902
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:33203
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:15 Aug 2012 18:28
Last Modified:14 Nov 2014 19:21

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